相关实验视频

Updated: Sep 12, 2025

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Published on: May 16, 2021

不同的禁食方法与跑步运动相结合,通过小鼠的AMPK/SIRT1/BDNF通路调节葡萄糖代谢

Xukai Wang¹, Jian Shi¹, Yinhuan Li¹

¹College of Food Science, The Key Laboratory of Food Quality and Safety of Guangdong Province, South China Agricultural University, Guangzhou, China.

Comprehensive Physiology

|August 6, 2025

概括

时间限制禁食 (TRF) 与运动相结合,比替代性禁食 (ADF) 更有效地调节葡萄糖代谢. 这种综合方法改善了葡萄糖的吸收和抗氧化能力,为代谢健康提供了优越的策略.

科学领域:

代谢生理学代谢生理学
运动科学运动科学
营养生物化学营养生物化学

背景情况:

间歇性禁食和运动是管理异常葡萄糖代谢的潜在辅助.
间歇性禁食和运动调节葡萄糖代谢的确切机制仍然不完全理解.

研究的目的:

研究间歇性禁食和运动对正常小鼠葡萄糖代谢的调节效应和基础机制.
为了比较时间禁食 (TRF) 和交替禁食 (ADF) 与运动相结合时的疗效.

主要方法:

六组C57BL/6雄性小鼠接受了为期6周的干预措施:交替禁食 (ADF),时间限制禁食 (TRF) 或控制,每组都与跑步运动相结合.
西方斑点分析用于评估主要代谢和信号通路中的蛋白质表达,包括AMPK,SIRT1,BDNF,MAPK和Nrf2.

主要成果:

运动的ADF和TRF都降低了糖化血清蛋白和肝糖原,同时增加了肌肉糖原.
禁食和运动可以调节AMPK,PGC-1α,Glut-4,SIRT1和PPAR-γ等蛋白质,并降低FoxO1的调节,从而增强葡萄糖吸收和脂质代谢.
与ADF相比,与运动相结合的TRF对这些代谢标志物和激活脑衍生神经营养因子 (BDNF) 和抗氧化途径 (MAPK,Nrf2/HO-1) 的效果显著更大.

结论:

限时禁食 (TRF) 在与运动相结合时,在调节葡萄糖代谢方面比替代性禁食 (ADF) 更有效.
联合禁食和运动干预措施显示,与禁食单独相比,新陈代谢调节更好.

关键词:

葡萄糖的新陈代谢.甘油脂代谢转换的代谢转换断断续续的禁食是一种禁食.跑步是一种炼.

更多相关视频

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT

Published on: January 7, 2018

相关概念视频

Glucose Homeostasis: Regulation of Blood Glucose

Glucose Homeostasis: Regulation of Blood Glucose

Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...

Metabolic States of the Body: Fasting and Starvation

Metabolic States of the Body: Fasting and Starvation

During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...

Metabolic States of the Body: The Postabsorptive State

Metabolic States of the Body: The Postabsorptive State

The postabsorptive state usually starts about four hours after a meal and lasts until the next meal is eaten. During this time, the digestive system stops absorbing nutrients, and the body uses stored energy reserves to maintain stable blood glucose levels.
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...