白尾鹿和鹿之间的差异 经过羊的经过后,慢性消耗疾病的病原体经过了羊
Alexis J Frese1,2,3, Eric D Cassmann4, Jifeng Bian1
1Virus and Prion Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United States.
Frontiers in veterinary science
|August 6, 2025
概括
白尾鹿的慢性衰竭病 (CWD) 对绵羊没有显著的风险. 然而,来自鹿 (MD) 的CWD对绵羊和鹿类都构成风险,这凸显了疾病监测的重要性.
科学领域:
- 兽医神经学 兽医神经学
- 子疾病研究研究 子疾病研究
- 野生动物流行病学
背景情况:
- 慢性衰竭疾病 (CWD) 是一种致命的病,影响像白尾鹿 (WTD) 和鹿 (MD) 这样的鹿类.
- 慢性病的物种间传播是可变的,受到许多因素的影响.
- 从鹿 (MD) 获得的CWD已被证明可以在内接种疫苗后传播给绵羊.
研究的目的:
- 为了评估羊在内接种疫苗后对WTD CWD病原体的敏感性.
- 通过在绵羊和转基因小鼠中的后续通道来表征WTD CWD病原体.
主要方法:
- 15只具有不同PRNP基因型的萨福克绵羊被内注射了WTD CWD剂.
- 西方斑块和EIA被用于检测羊脑和淋巴组织中的错误折叠蛋白 (PrPSc).
- 阳性绵羊大脑物质被传递给化 (Tg12) 和卵化 (Tg338) 的小鼠.
主要成果:
- 在15只接种疫苗的羊中,有2只的脑干 (ARQ/ARQ基因型) 检测到了PrPSc,平均潜伏期为39个月.
- 通过WTD绵羊CWD进入Tg12小鼠显示了83%的攻击率和377天的潜伏期;在Tg338小鼠中没有检测到PrPSc.
- 通过MD绵羊CWD进入Tg338小鼠显示了100%的攻击率和282天的潜伏期.
结论:
- 由于WTD CWD具有较低的传播到绵羊的风险.
- WTD CWD可能会传回鹿类种群.
- 绵羊的MDCWD对鹿类和绵羊种群都有潜在的风险,这强调了监测的必要性.
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