环境暴露对可转移元素的表观基因组和转录基因组景观的影响
Benpeng Miao1, Yu Zhang2,3, Wanqing Shao1
1Department of Genetics, Washington University School of Medicine, St. Louis, MO 63108, USA.
bioRxiv : the preprint server for biology
|August 6, 2025
概括
早期暴露于环境毒素会改变小鼠肝脏中的可移植元素 (TE),影响基因调节和免疫反应. 这些移动DNA序列显示出明显的,取决于性别的变化,揭示了新的健康风险.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 毒理学 毒理学 毒理学
背景情况:
- 可转移元素 (TE) 是移动DNA序列,包括哺乳动物基因组的很大一部分.
- TEs通常在表观遗传上被静止,但可以调节基因表达和染色素.
- 在环境暴露下,TEs的监管作用在很大程度上是未知的.
研究的目的:
- 调查小鼠肝脏中TEs的调节功能,在生命早期暴露于各种环境毒素后.
- 为了识别特定的可转移元素变化及其相关基因,以应对有毒物质暴露.
- 阐明早期暴露于有毒物质对TE调节和潜在健康后果的长期影响.
主要方法:
- 来自小鼠肝脏组织的351个多omics数据集的综合分析.
- 评估染色质可访问性和基因表达变化.
- 可转移元素 (TE) 和 TE 基因化学转录的识别.
主要成果:
- 早期暴露于有毒物质 (,,BPA,TBT,DEHP,TCDD,PM2.5) 改变了成年小鼠的染色质可访问性和TE表达.
- 观察到明显的暴露特异性和性别依赖的特征.
- 6,699个TEs显示染色质可访问性发生变化,其中LINE和LTR元素分别与代谢和免疫基因相关.
- 140个TE基因的化学转录表明了TE-贡献的转录可塑性.
结论:
- 早期暴露于环境中有毒物质会导致可移植元素的长期表观遗传变化.
- 改变的TE可以影响基因调节,特别是在代谢和免疫路径中.
- 这些发现强调了TE作为中介环境暴露的长期健康影响的关键参与者.
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