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针对HSV糖蛋白B的抗体需要效应器功能来保护新生小鼠
Matthew D Slein1,2, Lesle M Jiménez1,2, Iara M Backes1,2
1Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.
bioRxiv : the preprint server for biology
|August 6, 2025
概括
针对简单疹病毒 (HSV) 糖蛋白B (gB) 的单克隆抗体保护新生儿免受HSV感染. Fc效应器功能至关重要,AAV介导的抗体表达提供跨代保护,为疫苗开发提供信息.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 疫苗开发 疫苗开发
背景情况:
- 简单疹病毒 (HSV) 感染构成重大威胁,特别是在新生儿身上.
- 糖蛋白B (gB) 对于HSV病毒进入至关重要,也是免疫干预的关键目标.
- 了解针对gB的抗体机制对于有效的疫苗设计至关重要.
研究的目的:
- 调查gB特异性单克隆抗体 (mAbs) 对新生儿HSV感染的保护机制.
- 评估病毒中和和Fc效应器功能在mAb介导保护中的作用.
- 评估用于治疗和预防策略的体内抗体表达的潜力.
主要方法:
- 利用新生儿HSV感染的小鼠模型.
- 使用一组gB特异性单克隆抗体 (mAbs).
- 采用腺相关病毒 (AAV) 介导的gB特异性mAb.Ab体内表达.
主要成果:
- 病毒中和Fc效应函数都对mAb介导的防治HSV死亡率做出了贡献.
- Fc效应器的功能是剂量依赖的,对保护至关重要.
- 在gB特定的mAb的AAV介导的体内表达赋予了跨代保护,防止后代的HSV-1和HSV-2死亡率.
结论:
- 针对HSV gB的抗体是强大的治疗药物.
- 最佳的保护需要具有多种功能配置的抗体,包括Fc效应器功能.
- 特定于gB的mAbs及其体内表达的策略对预防和治疗HSV充满希望,指导未来的疫苗设计.
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