一个不寻常的遗传开关控制着Mycobacterium avium的发病,抗生素耐药性和殖民地形态
Hannah J Nilsson1, Zoe Netter1, Miles Tuncel2
1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
bioRxiv : the preprint server for biology
|August 6, 2025
概括
我的细菌 (Mycobacterium avium) 亚种 hominissuis 在毒性,抗生素耐药 (SmT) 和毒性,抗生素敏感 (SmO) 状态之间切换. 这种开关是由影响Erp蛋白的IS1245转移控制的,为抗感染提供了新的策略.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 病变的发生和发病.
背景情况:
- 菌 (Mycobacterium avium) 亚种Hominissuis (Mah) 是一种适应性强的环境病原体.
- 马赫表现出两种殖民地形态:光滑透明 (SmT) 和光滑不透明 (SmO).
- SmT细胞是毒性和抗生素耐药的,而SmO细胞是毒性,抗生素敏感,生长速度更快.
研究的目的:
- 阐明Mah的SmT和SmO殖民地形态之间的相互转换背后的机制.
- 为了确定控制这种表型切换的遗传因素.
- 为了探索Mycobacterium avium感染的潜在治疗点.
主要方法:
- 对IS1245转移事件的分析.
- 这是ERP基因和marP基因的基因操纵.
- 对殖民地形态和抗生素耐药性的评估.
主要成果:
- SmT-SmO切换是通过IS1245的可逆转移到erp基因来调节的.
- 细胞外重复蛋白 (Erp) 对于维持SmT状态至关重要,并调解从SmO转变为SmT.
- 在MarP蛋白酶中的突变将Mah锁定在SMO状态中,抑制Erp介导的切换.
结论:
- Erp和MarP是信号传导途径的关键组成部分,调节了Mah中对周等离子体应激的反应.
- 了解这些切换机制为打击Mycobacterium avium感染中的抗生素耐药性提供了潜在的新策略.
更多相关视频
07:10A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
9.1K
05:06Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024
727
相关概念视频
01:28Gene Regulation in Microbial Communities: Quorum Sensing
92
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
92
01:30Development of Antibiotic Resistance
203
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
203
01:16Transduction
112
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
112
00:57Antibiotic Selection
55.3K
Overview
55.3K
01:31Defense Against Bacterial Pathogens
1.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K