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Updated: Sep 12, 2025

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关于使用具有分散体内活体流细胞计的对比剂的考虑,以检测循环癌细胞群

Joshua S Pace¹, Grace Matheson¹, Gauri Malankar²

¹Northeastern University, Department of Bioengineering, Boston, MA, USA.

bioRxiv : the preprint server for biology

|August 6, 2025

概括

开发新的光对比剂用于体内循环瘤细胞 (CTC) 的体内清单,对于精确的癌症转移监测至关重要. 目前的药物表现有前途,但需要提高特异性,以避免来自非癌细胞的错误阳性.

科学领域:

在瘤学瘤学.
医疗成像医学成像
生物技术是生物技术.

背景情况:

由循环瘤细胞 (CTC) 驱动的转移是癌症死亡的主要原因.
目前使用血液样本的CTC计数方法通常不准确,无法跟踪随时间的变化.
流细胞计依赖于标记驱动的分析,用于CTC识别和计数.

研究的目的:

确定有效的CTC对比剂的特征,用于体内光成像.
评估候选对比剂在非侵入性CTC计数中的潜力.
评估光引导手术 (FGS) 应用中的对比剂.

主要方法:

评估了向叶酸受体 (OTL38),向泛素 (VGT-309) 和向PSMA的对比剂.
使用细胞培养模型在体外测试的药物.
使用小鼠模型在体内评估药物的性能.

主要成果:

所有测试的药物都显示了目标细胞系的高吸收率.
观察到非癌症血细胞的轻微但显著的标记.
快速清除和化方法减少了背景光.

结论:

目前的光对比剂显示出CTC标签的潜力,但缺乏足够的特异性.
血细胞的非特异性标记导致错误的阳性CTC计数.
改进的代理设计和多重复合可能会提高精确CTC检测的特异性.

关键词:

在循环中的瘤细胞中.化对比剂是一种化对比剂.在体内标记的标签.

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