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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Sep 12, 2025

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
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Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

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旋盘共聚焦显微镜用25兆像素摄像头

Guy M Hagen1, Brian Lewis1, Summer Levis1

  • 1UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, Colorado, 80918, USA.

bioRxiv : the preprint server for biology
|August 6, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种可负担的旋盘共聚焦显微镜系统,使用CMOS摄像头. 低成本的设置提供了高分辨率的3D成像,使先进的显微镜更容易获得.

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相关实验视频

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科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 显微镜技术 显微镜技术
  • 光学工程是指光学工程.

背景情况:

  • 旋盘共聚焦显微镜提供快速的光学切割和低光毒性.
  • 高昂的硬件成本限制了先进的旋盘共聚焦显微镜系统的可用性.

研究的目的:

  • 开发一个低成本的旋盘共聚焦显微镜系统.
  • 为了证明在旋盘显微镜中使用负担得起的CMOS摄像机的可行性.
  • 为先进的生物成像提供开源和可扩展的解决方案.

主要方法:

  • 使用了一个25兆像素的机器视觉CMOS摄像头 (索尼IMX540,FLIR Blackfly S).
  • 设计和制造了一个定制的旋转盘,有18微米的针孔.
  • 整合了摄像头和磁盘与光学机电机和微型管理器软件.

主要成果:

  • 实现了生物样本的高分辨率3D成像 (老鼠大脑切片,老鼠丸).
  • 经过验证的性能高量子效率 (69%在525nm) 和低读音噪声 (2.31电子) CMOS传感器.
  • 证明了稳定的,接近远程中心的成像能力.

结论:

  • 价格实惠的CMOS传感器可以有效地取代旋盘显微镜中的sCMOS摄像头.
  • 开发的系统为先进的成像提供了一个具有成本效益的替代方案.
  • 开源性质促进了研究中的可扩展性和更广泛的采用.