Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

HSV-1 and VZV co-reactivation: implications for worsening neurological and neurodegenerative diseases.

Journal of neurovirology·2026

Same author

Multi-site temporal control of optogenetic stimulation enhances firing frequencies in peripheral nerves.

bioRxiv : the preprint server for biology·2026

Same author

Intranasal HSV-1 Infection Drives Region-Specific Interferon-Dominant Microglial Remodeling.

bioRxiv : the preprint server for biology·2026

Same author

High-speed in vivo calcium recording using structured illumination with self-supervised denoising.

Optics continuum·2026

Same author

Miniaturized widefield microscope for high speed in vivo voltage imaging.

Biomedical optics express·2026

Same author

Voltage imaging with periodic structured illumination.

Biomedical optics express·2026

Search research articles

相关实验视频

Updated: Sep 12, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

使用周期结构照明进行电压成像.

Forest Speed¹, Alec Teel², Gregory L Futia¹

¹Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.

bioRxiv : the preprint server for biology

|August 6, 2025

概括

此摘要是机器生成的。

我们引入伪HiLo (pHiLo) 成像,通过减少失焦信号来改善体内电压成像. 这种技术提高了研究清醒小鼠神经活动的清晰度.

更多相关视频

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F⁻

Published on: July 27, 2018

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

相关实验视频

Last Updated: Sep 12, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F⁻

Published on: July 27, 2018

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

科学领域:

神经科学是一个神经科学.
光学成像技术的成像
生物物理学的生物物理.

背景情况:

在体内电压成像对于理解神经电路动态至关重要.
失焦的光会污染传统广场录音中的信号.
提高信号清晰度对于高速神经活动研究至关重要.

研究的目的:

评估周期结构化照明的有效性,使用伪HiLo (pHiLo) 图像重建进行体内电压成像.
将pHiLo与传统的广场和伪广场 (pWF) 方法进行比较,以减少失焦信号污染.
分析与pHiLo相关的权衡,用于在清醒的小鼠中进行高速成像.

主要方法:

使用周期结构化照明与伪HiLo (pHiLo) 图像重建.
进行了体内电压成像实验.
将pHiLo重建与传统的广场记录和伪广场 (pWF) 重建进行比较.

主要成果:

与传统的广场录音相比,pHiLo显著降低了失焦细胞的信号.
减少了失焦信号污染,改善了来自聚焦电池的电压活动的清晰度.
在醒着的小鼠的高速电压成像中展示了pHiLo的应用.

结论:

pHiLo图像重建是增强体内电压成像的有效方法.
pHiLo提供了通过最小化失焦光来改善信号与背景的比率.
对信号噪声比和时间分辨率权衡的进一步调查是必要的,以优化pHiLo在高速应用中.