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使用周期结构照明进行电压成像

Forest Speed¹, Alec Teel², Gregory L Futia¹

¹Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.

bioRxiv : the preprint server for biology

|August 6, 2025

概括

我们引入伪HiLo (pHiLo) 成像,通过减少失焦信号来改善体内电压成像. 这种技术提高了研究清醒小鼠神经活动的清晰度.

科学领域:

神经科学是一个神经科学.
光学成像技术的成像
生物物理学的生物物理.

背景情况:

在体内电压成像对于理解神经电路动态至关重要.
失焦的光会污染传统广场录音中的信号.
提高信号清晰度对于高速神经活动研究至关重要.

研究的目的:

评估周期结构化照明的有效性,使用伪HiLo (pHiLo) 图像重建进行体内电压成像.
将pHiLo与传统的广场和伪广场 (pWF) 方法进行比较,以减少失焦信号污染.
分析与pHiLo相关的权衡,用于在清醒的小鼠中进行高速成像.

主要方法:

使用周期结构化照明与伪HiLo (pHiLo) 图像重建.
进行了体内电压成像实验.
将pHiLo重建与传统的广场记录和伪广场 (pWF) 重建进行比较.

主要成果:

与传统的广场录音相比,pHiLo显著降低了失焦细胞的信号.
减少了失焦信号污染,改善了来自聚焦电池的电压活动的清晰度.
在醒着的小鼠的高速电压成像中展示了pHiLo的应用.

结论:

pHiLo图像重建是增强体内电压成像的有效方法.
pHiLo提供了通过最小化失焦光来改善信号与背景的比率.
对信号噪声比和时间分辨率权衡的进一步调查是必要的,以优化pHiLo在高速应用中.

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