早期接触会使后代患喘风险增加:转录组和表观基因组分析
bioRxiv : the preprint server for biology
|August 6, 2025
概括
母亲接触饮用水中的无机 (iAs) 可能会增加后代喘风险. 这种表观遗传重编程影响基因表达和DNA甲基化,可能导致过敏呼吸道过敏反应.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 饮用水中的无机 (iAs) 构成全球健康风险.
- 表观遗传机制对于调解环境暴露和疾病发展至关重要.
研究的目的:
- 调查世卫组织暂定水平 (10微克/升) 的母亲暴露在无机 (iAs) 中是否会增加后代喘风险.
- 探索表观遗传重编程,特别是DNA甲基化和microRNA变化的作用,以调解这种风险.
主要方法:
- F1小鼠在产前暴露于饮用水中的IA.
- 在5个月的血液样本上进行了转录组和甲基组分析.
- 试验小鼠使用家用过敏原,然后进行肺功能测试.
主要成果:
- 产前IA暴露导致呼吸道过敏反应 (AHR) 的增加和炎症基因表达的改变.
- 观察到显著的DNA甲基化变化,包括miR-101c的下调.
- 在胎儿和成人肺组织,羊水和血液中,miR-101c下调持续存在,与过敏AHR和TGFβ通路失调相关.
结论:
- 通过表观遗传变化,母亲对IA的暴露可以通过表观遗传变化为后代引发喘发育.
- 这些发现凸显了IA破坏正常发育并增加对过敏疾病的易感性的潜力.
- 这项研究表明,miR-101c和TGFβ途径是iAs诱导喘风险评估的潜在生物标志物.
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