Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

19.3K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
19.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Peer influence decay and behavioral diffusion in adolescent networks: A simulation approach.

Science (New York, N.Y.)·2026
Same author

Jasmonate signaling and prey nutrient availability trigger distinct biochemical responses in the Drosera capensis feeding cycle.

Plant physiology·2026
Same author

Design and Analysis of Untargeted Metabolomics Experiments.

Current protocols·2025
Same author

Jasmonate-induced prey response in the carnivorous plant <i>Drosera capensis</i>.

bioRxiv : the preprint server for biology·2025
Same author

Mini-αA-crystallin protects a client lens protein from catastrophic aggregation due to heat stress.

Protein science : a publication of the Protein Society·2025
Same author

Endogenous competition and the underrealized reproduction of infectious diseases.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Leveraging target enrichment and genome skimming (Hyb-Seq) of herbarium collections to unlock timber DNA barcoding.

Applications in plant sciences·2026
Same journal

Detecting cryptic ghost lineage introgression in four-taxon genomic datasets.

Applications in plant sciences·2026
Same journal

HapAsmbl: A reference-aided pipeline for assembling haplotypes in Nanopore amplicon sequence data of polymorphic populations.

Applications in plant sciences·2026
Same journal

HybSuite: An integrated pipeline for hybrid capture phylogenomics from reads to trees.

Applications in plant sciences·2026
Same journal

Detecting introgression from phylogenetic invariant site patterns using machine learning.

Applications in plant sciences·2026
Same journal

tanggle: An R package for the visualization of phylogenetic networks.

Applications in plant sciences·2026
查看所有相关文章

相关实验视频

Updated: Sep 12, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.5K

计算机辅助序列注释 (CASA) 工作流程用于酶发现.

Gemma R Takahashi1, Franchesca M Cumpio1, Carter T Butts2

  • 1Department of Molecular Biology and Biochemistry University of California Irvine 92697-3900 California USA.

Applications in plant sciences
|August 6, 2025
PubMed
概括
此摘要是机器生成的。

从大量的序列数据中识别合适的酶候选者是具有挑战性的. 计算机辅助序列注释 (CASA) 提供了详细的序列和结构信息,以帮助选择蛋白质进行进一步的生物化学研究.

关键词:
德罗塞拉本斯 (Drosera capensis) 是一个长毛虫.发现了酶的发现.基因组注释 基因组注释蛋白质酶蛋白质酶是一种蛋白质.蛋白质序列分析分析蛋白质序列分析蛋白质序列注释注释

更多相关视频

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

730
Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

361

相关实验视频

Last Updated: Sep 12, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.5K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

730
Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

361

科学领域:

  • 生物化学 生物化学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 越来越多的核酸测序数据在酶发现方面提出了挑战.
  • 目前的注释方法通常仅依赖于序列相似性,这不足以选择有前途的酶候选者.
  • 详细的序列和结构信息对于有效的酶表征至关重要.

研究的目的:

  • 为增强蛋白质注释引入一种新的计算工作流.
  • 为了使酶发现,自动化新型蛋白质表征的关键方面.
  • 为了生成超出基本序列相似性的人类可解释的注释.

主要方法:

  • 开发计算机辅助序列注释 (CASA),一个基于Python的工作流.
  • 自动生成高度信息化和丰富的注释序列对齐.
  • 使用Drosera capensis*基因组中的酶进行演示.

主要成果:

  • CASA制作详细的注释,将新奇的序列与已知的引用进行比较.
  • 附注包括预测的功能,活性位点残留物,二硫化物键和基质结合位点.
  • 工作流成功地表征了一种来自Drosera capensis*的酶.

结论:

  • 详细的注释和蛋白质结构预测对于从序列数据中选择酶点至关重要.
  • CASA有助于识别适合生物化学和结构生物学的蛋白质标.
  • 该CASA工具链是免费可用的,促进在酶发现更广泛的应用.