素-6-酸盐合成酶VvTPS10通过参与CBF通路和葡萄藤中VvPUB19介导的乌比基因化来调节耐寒性
Mengyu Zhao1,2, Ling Wang3, Meijuan Feng2,4
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Plant, cell & environment
|August 6, 2025
概括
葡萄酒II类三糖6酸合成酶 (VvTPS10) 通过调节CBF通路和糖糖代谢来增强耐寒性. 这项研究揭示了VvTPS10作为植物抗寒机制的关键调节器.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 环境压力生理学生理学
背景情况:
- 寒冷压力显著影响葡萄藤生理学和行业的可持续性.
- 二级三六合成酶 (TPS) 对植物应激反应和糖代谢至关重要,但它们的抗寒作用尚不清楚.
- 了解VvTPS10的功能对于改善葡萄树寒冷耐受性至关重要.
研究的目的:
- 在葡萄藤中阐明冷诱导性II类TPS基因VvTPS10的抗寒功能.
- 调查VvTPS10介导的寒冷耐受性背后的监管机制.
主要方法:
- 葡萄树中的转基因技术 (基因沉默和过度表达).
- 生物化学试验包括蛋白质相互作用和无处不在试验.
- 转录组分析和促进体结合试验.
主要成果:
- 沉默VvTPS10增加了寒冷敏感性,而过度表达增加了寒冷耐受性和三糖含量.
- VvTPS10通过CBF通路运行,其中VvCBF1通过转录激活VvTPS10.
- VvPUB19通过ubiquitination负面调节VvTPS10的稳定性;VvTPS10与VvSnRK1相互作用并影响糖糖代谢.
结论:
- VvTPS10是一种新型阳性调节器,用于葡萄葡萄中耐寒的调节器.
- VvTPS10通过CBF通路集成冷信号,并影响糖代谢.
- 这项研究提供了对II类TPS功能的新见解以及葡萄树冷性策略的新见解.
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