来自越南本土不同豆类的酶蛋白化物的结构,功能和生物特性对比研究
Thi-Thuy-Dung Nguyen1, Thanh-Sang Nguyen2,3, Thi-Van-Linh Nguyen1
1Faculty of Applied Science and Technology (FAST), Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam.
Applied biochemistry and biotechnology
|August 6, 2025
概括
豆类蛋白质的酶性水解,如黄色,黄和利马豆的蛋白质,显著增加了抗氧化和发泡的特性. 这些植物性蛋白质水解剂不表现出细胞毒性,为健康,可持续的食品产品提供了潜力.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 营养保健品 营养保健品
背景情况:
- 越来越多的消费者对植物性蛋白质的需求,以促进健康和可持续发展.
- 水解蛋白质比原始蛋白质提供了更好的功能和生物特性.
- 豆类是植物性蛋白质的宝贵来源.
研究的目的:
- 为了研究使用Alcalase的酶化水解对豆类蛋白质特性的影响.
- 评估黄色豆,黄豆和豆蛋白质水解剂的功能,技术和生物活动.
- 评估抗氧化能力,发泡性质,形状和细胞毒性.
主要方法:
- 使用Alcalase的黄色豆,黄豆和豆蛋白质的酶性水解.
- 对抗氧化活性的测定 (ABTS激素清除,FRAP).
- 评估发泡能力,SDS-PAGE分析大小,和细胞毒性测试.
主要成果:
- 水解显著增强了所有豆类蛋白质水解物的抗氧化活性.
- 黄豆水解剂显示出最高的抗氧化活性 (ABTS:754.85毫克TE/L,FRAP:80.35毫克TE/L).
- 黄豆蛋白具有最高的水解度 (81.38%) 和改善的泡特性.
- SDS-PAGE证实了蛋白质降解为低分子量 (15-45 kDa).
- 在2000微克/毫升以下没有观察到细胞毒性.
结论:
- 酶性水解有效地改善豆类蛋白的功能和生物特性.
- 豆类蛋白质水解剂具有强大的抗氧化和功能性活性.
- 这些水解剂是可持续的,促进健康的食品和营养品的有希望的多功能成分.
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