分裂-YFP-合的相互作用依赖的TurboID识别了Arabidopsis基底细胞极性的新功能
Aobo Huang1,2, Jinming Zhang1,2, Zhendong Liu1
1The Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
概括
研究人员使用一种新型的近距离标记系统确定了参与植物细胞极性作用的新蛋白质. 该系统有助于了解植物如何建立身体轴,并影响生长,将发育规划与环境反应联系起来.
科学领域:
- 植物生物学 植物生物学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 身体轴的形成对于多细胞生物的发展至关重要.
- 门口蛋白BASL (在门口系中突破不对称性) 参与建立Arabidopsis叶, hypocotyls 和根的细胞极性.
研究的目的:
- 开发一种新的近距离标记系统,SSYID (Split-YFP和TurboID的协同融合),用于识别涉及组织范围细胞极性中的蛋白质相互作用.
- 揭示细胞极性模块的分子组成部分,其中包括Arabidopsis中的BASL和BREVIS RADIX-LIKE 2 (BRXL2) 蛋白质.
主要方法:
- 开发并使用szyd系统用于靠近依赖的生物化.
- 结合近距离标记与质谱测量来识别相互作用的蛋白质.
- 通过淘汰AGC蛋白激酶同类物进行基因分析.
主要成果:
- 鉴定了一种涉及BASL和BRXL2蛋白质的全组织细胞极性模块.
- 发现与此模块相关的未表征的蛋白质候选物.
- 发现一个AGC蛋白激酶局限于基底膜,其淘汰会影响皮细胞的生长,这表明与植物向上生长的联系.
结论:
- tSYID系统有效地识别了靠近蛋白质活性位点的近邻蛋白质.
- 证明了基底细胞极性与Arabidopsis.植物上升生长之间的联系.
- 突出了发育身体轴与植物环境生长线索之间的相互作用.
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