不混合的蛋白质在RNA结合中竞争,以订购凝结层
Wilton T Snead1,2, Mary K Skillicorn3,4, Krishna Shrinivas3,4
1Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611.
概括
核斑通过竞争性RNA结合来组织. 像FUS和NONO这样的核心蛋白质与外相关的NEAT1RNA结合,挑战了以前的模型并揭示了生物分子凝聚物组织的原理.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- 生物分子凝结物分隔细胞功能.
- 许多凝结物具有独特的内部子域.
- 了解控制凝结物组织的规则至关重要.
研究的目的:
- 为了研究核的组装逻辑.
- 为了确定 lncRNA NEAT1 支架如何构成 paraspeckle 子域.
- 阐明FUS,NONO和TDP-43在抛光镜组织中的作用.
主要方法:
- 对RNA-蛋白相互作用的生物信息学分析.
- 生物化学实验研究蛋白质结合偏好.
- 凝聚剂组装的基于物理的模拟.
主要成果:
- 核心蛋白FUS和NONO优先结合与外相关的NEAT1域,这与当前流行的模型相反.
- 贝蛋白TDP-43在核心蛋白质凝聚物周围形成类似表面活性剂的层.
- 竞争性RNA结合和蛋白质不混合性驱动了对光斑层的形成.
结论:
- 抛光镜组织是由核心蛋白质与特定NEAT1RNA域的竞争性结合控制的.
- 附加组织可以从协作和竞争互动的平衡中出现.
- 这项研究重新定义了对 lncRNA 中介生物分子凝聚聚合物的理解.
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