Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Scaling and self-similarity in the formation of the embryonic epigenome.

Nature physics·2026

Same author

Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells.

Development (Cambridge, England)·2026

Same author

Cell-to-cell variability and gain of methylation at polycomb CpG islands as a hallmark of aging.

Nature communications·2026

Same author

LIPID MAPS: Powering discovery in lipidomics.

Science signaling·2026

Same author

Using CRISPR barcoding as a molecular clock to capture dynamic processes at single-cell resolution.

Genome research·2026

Same author

DNMT1 loss leads to hypermethylation of a subset of late replicating domains by DNMT3A.

PLoS genetics·2026

Search research articles

相关实验视频

Updated: Jun 14, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

使用scMTR-seqq在单细胞中对多个基因组修饰和转录组进行组合分析.

Yang Wang¹, Jingyu Li¹, Andrew A Malcolm¹

¹Epigenetics Programme, Babraham Institute, Cambridge CB22 3AT, UK.

Science advances

|August 6, 2025

概括

一种新的方法,单细胞多标和mRNA测序 (scMTR-seq),同时对单细胞中的六种基因组修饰和转录组进行了分析. 这项技术揭示了人类内皮分化和小鼠胚胎发育过程中的动态表观遗传变化.

更多相关视频

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

相关实验视频

Last Updated: Jun 14, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

科学领域:

表观遗传学和基因组学
细胞和分子生物学细胞和分子生物学
发展生物学发展生物学

背景情况:

在复杂样本中绘制染色质状态的图表是具有挑战性的,因为缺乏在单细胞中同时分析多个组质子修饰和转录组的方法.
了解组合性组织蛋白修饰对于破译基因调节元素和表观遗传程序至关重要.
之前的方法不足以在单细胞水平上进行高通量,多原子的分析.

研究的目的:

开发和验证一种新的高通量方法,用于在单细胞中同时分析多个基因组修饰和转录组.
调查在人类内皮分化过程中的动态色素状态和转录组变化.
在小鼠胚胎囊中生成由谱系解析的染色质图和基因调节网络.

主要方法:

开发单细胞多目标和mRNA测序 (scMTR-seq).
scMTR-seq的应用,在单细胞中同时对六种基因组修饰和转录组进行分析.
使用scMTR-seq数据分析人类内皮分化和小鼠胚胎细胞的发展.

主要成果:

在单细胞中,scMTR-seq成功启用了六种基因组修饰和转录组的同时分析.
在人类内皮分化过程中发现了染色质状态和转录组的动态和协调变化.
在小鼠胚芽细胞中制造出血统解析的染色质地图和基因调节网络,揭示了表观遗传不对称性,并将Trps1确定为潜在的抑制剂.

结论:

scMTR-seq是一个强大的工具,用于调查异质样本中的组合色素景观.
该方法为表观遗传调节系统和发育过程中的基因调节提供了新的见解.
scMTR-seq有助于更深入地了解动态表观遗传变化和谱系规范.