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能量化结构水对H9c2细胞中生物能量功能和氧化应激的保护作用

Sachithra S Ranaweera¹, Juan P Zuniga-Hertz², Ramamurthy Chitteti²

¹Department of Anesthesiology, University of California, San Diego, La Jolla, United States.

Explore (New York, N.Y.)

|August 6, 2025

概括

活性化结构水 (ESW) 增强了H9c2细胞的细胞能量生产和抗压能力. 治疗ESW改善了线粒体功能,减少了氧化损伤,这表明潜在的健康益处.

科学领域:

细胞生物学细胞生物学
生物能源学生物能源学
氧化压力研究研究氧化压力研究

背景情况:

能量结构水 (ESW) 的概念通过能量频率提出了健康益处,但机制仍然不清楚.
了解ESW对细胞功能的影响对于验证其所谓的影响至关重要.

研究的目的:

研究ESW对H9c2细胞中细胞生物能量功能的影响.
评估ESW在减轻氧化应激方面的作用及其对细胞活力的影响.
通过基因表达分析识别ESW调节的分子通路.

主要方法:

用ESW或对照水 (CW) 处理H9c2细胞.
使用Agilent海马XF Pro分析仪测量了线粒体功能.
在氧化应激下评估了细胞活力和反应性氧物种 (ROS) 生产.
进行了差异基因表达和基因本体学 (GO) 丰富分析.

主要成果:

通过ESW预处理,可以显著提高最大和备用呼吸能力.
在没有改变线粒体或总ATP水平的情况下,ESW增加了糖溶性ATP的产生.
在H2O2诱导的氧化应激下,ESW增强了细胞活力并减少了ROS的产生.
基因表达分析揭示了各种基因的显著上调和下调,GO分析突出了对细胞应激反应途径的影响.

结论:

治疗ESW增强了细胞的生物能量,特别是增加了呼吸能力和糖溶性ATP的产生.
ESW 增强了对氧化应激的抵抗力,并提高了细胞活力.
ESW调节与细胞应激反应和能量代谢相关的基因表达,这表明其观察到的影响的潜在机制.

关键词:

生物能源学生物能源学细胞活力细胞活力充电结构化水具有活力的结构.基因表达分析基因表达分析线粒体呼吸线粒体呼吸氧化应激是一种氧化应激.

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