素通过通过ROS/JNK/p53轴对抗亡来抑制PEDV复制
Yupeng Zhi1, Yupeng Ren1, Xuemei Xia1
1Key Laboratory of Veterinary Medicine in Universities of Sichuan Province, Southwest Minzu University, Chengdu, 610041, China.
Microbial pathogenesis
|August 6, 2025
概括
素有效地通过减少病毒复制和亡来抑制猪流行性腹病毒 (PEDV). 这种黄类化合物向ROS/JNK/p53信号轴,为PEDV感染提供了潜在的新治疗策略.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 猪流行性腹病毒 (PEDV) 对猪产业构成重大威胁.
- 目前的免疫策略受到病毒进化和缺乏有效治疗方法的限制.
- 黄酸对PEDV的抗病毒潜力是公认的,但其机制尚未完全理解.
研究的目的:
- 为了验证辛对PEDV的抑制作用.
- 阐明素对抗PEDV感染的特定抗病毒机制.
- 为了研究素对参与PEDV诱导的亡的细胞通路的调节.
主要方法:
- 免疫光检测 (IFA) 和定量PCR (qPCR) 测试,以确认素的抗病毒活性.
- 感染PEDV的细胞的转录基因组测序,有或没有素处理.
- KEGG通路分析,反应性氧物种 (ROS) 水平评估,亡试验,关键蛋白质的西部涂抹和抑制剂研究 (SP600125,PFT-α).
主要成果:
- 氨酸显著抑制PEDV复制并降低病毒载量.
- 转录组分析揭示了素对与亡,先天免疫和细胞代谢相关的途径的影响.
- 氨酸通过调节ROS/JNK/p53信号轴,降低分裂的酶水平和Bax/Bcl-2比率来降低PEDV诱导的ROS产生和亡.
- 基因和p53的抑制剂模仿了素的抗病毒作用.
结论:
- 素对抗PEDV诱导的亡,并通过ROS/JNK/p53信号通路抑制病毒复制.
- 这项研究为开发素作为一种针对PEDV的新型治疗剂提供了机制基础.
- 研究结果提供了对PEDV和相关病毒感染的潜在抗病毒药物点的见解.
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