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解读肺结核和GERD之间的遗传重叠,以发现药物标:一个结构生物信息学观点

Sanjukta Dasgupta¹, Bishnupriya Saha², Sushmita Roy Chowdhury³

¹Department of Biotechnology, Brainware University, Barasat, India; Center for Multidisciplinary Research & Innovations, Brainware University, Barasat, India.

Microbial pathogenesis

|August 6, 2025

概括

这项研究揭示了肺结核 (PTB) 和胃食道逆流病 (GERD) 之间的共同分子通路. 综合生物信息学确定了共同的基因和microRNAs,为这两种疾病提出了利巴维林等新型治疗点.

科学领域:

生物信息学和分子生物学
基因组学和转录基因组学
药理学和药物发现

背景情况:

肺结核 (PTB) 和胃食道逆流病 (GERD) 是不同的临床实体.
新出现的证据表明,PTB和GERD之间可能存在共同的分子基础.
了解这些常见机制可能会揭示新的治疗策略.

研究的目的:

用综合生物信息学识别PTB和GERD中常见的分子机制和失调基因.
确定关键的调节性microRNA和两种疾病共享的信号通路.
探索治疗PTB和GERD的潜在药物重新利用候选药物.

主要方法:

基因表达数据的综合生物信息学分析.
鉴定显著失调的基因和调节性微RNA.
路径丰富分析和枢纽基因鉴定.
定量实时PCR用于基因表达验证.
在的药物重新利用和分子对接模拟.

主要成果:

确定了23个PTB和GERD常见的显著失调的基因.
突出显示的关键微RNAs (hsa-miR-34a-5p,hsa-let-7b-5p,hsa-let-7g-5p) 和与巨细胞相关的途径.
干扰素α/β信号传递成为一个核心的共享途径.
五个枢纽基因 (MYL9,OASL,ACTA2,DDX60L,DDX60) 被确定并验证.
利巴维林被预测为一个有前途的药物候选人,以有利的结合亲和力和ADMET特性准OASL.

结论:

PTB和GERD共享共同的分子路径和调节元素.
干扰素α/β信号传递和特定的枢纽基因代表了关键的分子链接.
利巴维林显示出作为重定向药物的潜力,用于向PTB和GERD的共享机制.
这些发现为开发针对这两种疾病的新,有针对性的治疗策略提供了基础.

关键词:

不同基因表达的差异性胃食道逆流疾病是胃食道逆流疾病.枢纽基因枢纽基因枢纽基因分子对接和模拟模型肺结核是一种肺结核.

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