细胞系和初级培养用于针对鱼类细菌病原体的疫苗开发:系统性审查
Nicolás Salinas-Parra1, Débora Torrealba2, Andrea Ahumada3
1Laboratorio de Genética y Genómica Aplicada, Escuela de Ciencias Del Mar, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330, Valparaíso, Chile.
Fish & shellfish immunology
|August 6, 2025
概括
细胞培养对于开发针对细菌病原体的鱼类疫苗非常有价值,有助于测试疗效和免疫反应. 然而,生物库的有限可用性阻碍了在水产养殖中广泛使用和可复制性.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 免疫学 免疫学 免疫学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 鱼类水产养殖中的细菌爆发导致大量的死亡率和经济损失.
- 在水产养殖中过度使用抗生素引发了人们对耐药性和环境影响的担忧.
- 疫苗开发对于可持续的养鱼和粮食安全至关重要.
研究的目的:
- 系统地审查目前关于细胞系和初级培养的知识,以开发鱼类疫苗.
- 确定细胞类型及其在制造针对细菌鱼类病原体的疫苗中的应用.
- 评估细胞培养模型在促进鱼类健康和水产养殖实践方面的有用性.
主要方法:
- 在Web of Science,PubMed和Scopus数据库中进行了系统的文献搜索.
- 在研究选择和数据提取方面遵循PRISMA指南.
- 纳入标准侧重于与开发针对细菌病原体的鱼类疫苗相关的研究.
主要成果:
- 确定了16个细胞系和4种初级培养物,用于鱼类疫苗开发的潜在用途.
- 主要应用包括评估免疫刺激作用,细胞毒性和DNA疫苗制造.
- 在主要生物库 (ATCC,JCRB,ECACC) 发现细胞系的可用性有限,影响了可复制性.
结论:
- 细胞系和初级培养显示出开发针对细菌疾病的有效鱼类疫苗的巨大潜力.
- 这些模型目前已在有限数量的鱼类物种中作为常规工具建立.
- 提高生物库中细胞系的可用性对于提高可再生性和推进鱼类疫苗研究至关重要.
相关概念视频
01:16Cell Lines
8.2K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
8.2K
01:21Cell Culture
18.6K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
18.6K
01:29Microorganisms in Medicine and Therapeutics
337
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
337