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智能微流体装置用于多重检测和上下呼吸道感染的及时预警
Shijue Gao1, Lei Wang2, Meng Zhao1
1National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Journal of advanced research
|August 6, 2025
概括
一种新的便携式诊断设备能够使用实时复合酶辅助放大 (RAA) 试验快速多重检测15种呼吸道病原体. 该工具为感染提供早期预警,特别是在资源有限的环境中.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 微流体学 微流体学
- 分子生物学分子生物学
背景情况:
- 呼吸道疾病的发病率和同时感染率不断上升,需要先进的多重诊断方法.
- 当前的诊断方法往往缺乏及时干预所需的速度和全面性.
研究的目的:
- 开发一个集成的, point-of-care 多重诊断工具,用于呼吸道病原体.
- 建立一个连接的呼吸道感染监测和预警平台.
主要方法:
- 开发一种便携式的微流体装置,使用实时复合酶辅助放大 (RAA) 试验.
- 自动化样本到答案诊断,包括核酸提取和检测.
- 使用427个样本对传统方法进行临床验证和早期预警平台的评估.
主要成果:
- 该设备在30-60分钟内实现了样本到答案诊断,识别了15种常见的呼吸道病原体.
- 证明了高灵敏度 (0.1 TCID50/mL对于病毒,10 CFU/mL对于细菌/真菌) 和准确性 (99.30%).
- 成功与智能监控系统集成,用于数据可视化和预警分析.
结论:
- 开发的设备提供了一个智能,便携式,快速和敏感的平台,用于早期诊断多种呼吸道病原体.
- 这项技术对于资源有限的环境特别有价值,改善了传染病监测.
- 综合系统有助于及时应对呼吸道疫情的公共卫生反应.
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