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Updated: Sep 12, 2025

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[基于CRISPR/dCas9系统的Sox17激活载体的构建及其在绵羊胚胎干细胞中的验证]

Wenli Lü1,2, Hua Yang1,2, Hui Xu1,2

  • 1Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|August 6, 2025
PubMed
概括

使用CRISPR/dCas9系统,电穿孔有效地激活羊胚胎干细胞 (sESC) 中的Sox17基因. 这种方法为sESC研究和细胞命运调节提供了一个稳定的基因激活协议.

关键词:
在CRISPR/dCas9中使用.这里是Sox17的位置.细胞的转化是细胞的转化胚胎干细胞是一种胚胎干细胞.基因编辑 基因编辑

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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