在乙乙尼利德应激下,Blakeslea trispora的适应性实验室进化导致增强β-胡卜素生物合成
Hamideh Moradi1, Davood Zare2, Mehrdad Azin1
1Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran.
Scientific reports
|August 6, 2025
概括
适应性实验室进化使Blakeslea trispora中的β-胡卜素产量增加了45%. 这种方法有效地增加了微生物生产这种有价值的营养化合物.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 布莱克斯莱亚三胞胎是天然β-胡卜素的关键微生物来源.
- 贝塔胡卜素具有重要的营养和工业价值.
- 提高微生物生产有价值的化合物对于工业应用至关重要.
研究的目的:
- 使用适应性实验室进化 (ALE) 来增加Blakeslea trispora中的β-胡卜素产量.
- 为了研究化学压力对真菌代谢物产生的影响和应激耐受性.
主要方法:
- 采用适应性实验室进化 (ALE) 策略,超过95次连续转移16个月.
- 暴露Blakeslea trispora菌株,以增加度的乙乙尼化物,一个生物合成的压力.
- 使用定量RT-PCR分析关键胡卜素基因的表达.
主要成果:
- 与野生类型 (21.6 mg/L) 相比,适应菌株A278 (54 mg/L) 的β-胡卜素产量增加了45%.
- 在适应菌株中观察到关键胡卜素基因 (hmgR,carRA,SR5AL) 的上调调节.
- 证明化学压力有效地增强了线状真菌中的代谢物产量和应激耐受性.
结论:
- 适应性实验室进化是改善Blakeslea trispora中的β-胡卜素产量的可行策略.
- ALE方法提高了真菌的应激耐受性和代谢物生产率.
- 这项研究为开发用于β-胡卜素生产的改良工业菌株提供了基础.
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