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非热大气等离子体通过ROS/WNT信号轴诱导骨质细胞中的骨质性活性

Ashish Ranjan Sharma¹, Nagendra Kumar Kaushik², Tirtha Raj Acharya²

¹Institute for Skeletal Aging & Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon 24341, South Korea.

ACS applied materials & interfaces

|August 7, 2025

概括

介质的非热等离子体 (NTP) 治疗通过增强骨形成标记物和原蛋白生产来刺激骨质母细胞活动. 这一过程涉及活性氧物种 (ROS) 和WNT信号通路,为骨再生提供了潜在的治疗策略.

科学领域:

生物医学工程生物医学工程
等离子体物理学的物理学
细胞生物学细胞生物学

背景情况:

非热等离子体 (NTP) 显示出对生物应用的前景.
尽管如此,NTP对骨质细胞活动的影响及其机制仍然不清楚.
研究NTP对骨质生成活性的影响对于治疗开发至关重要.

研究的目的:

为了研究NTP处理的介质在骨质母细胞上的骨质生成潜力.
阐明潜在的机制,包括信号通路和活性氧物种 (ROS).
探索NTP作为骨形成的治疗策略.

主要方法:

用介电屏障放电 (DBD) 和喷射NTP (JNTP) 处理细胞培养基.
骨质母细胞对NTP处理介质 (JNTPM) 的间接暴露.
分析ALP活动,骨质基因表达,原蛋白生产,矿化,WNT通路激活和ROS水平.

主要成果:

用JNTP处理的介质 (JNTPM) 显著诱导了骨质母细胞中的ALP活性和骨质原标记表达.
JNTPM增强了原蛋白的产生,矿化和WNT信号通路的激活 (β-catenin稳定,GSK3-β酸化).
随着JNTPM治疗,细胞内ROS水平增加,ROS抑制降低了WNT信号和骨质生成活性.

结论:

在JNTPM治疗刺激骨质细胞的骨质生成活性.
骨质效应是由ROS和WNT信号通路之间的交叉对话介导的.
用NTP处理的介质为增强骨形成提供了潜在的治疗方法.

关键词:

通过WNT信号通路传递信号.非热等离子体的非热等离子体.骨质母细胞 (osteoblasts) 是一个骨质细胞.骨质性活动的骨质性活动.有活性氧物种的反应性氧物种.

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