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几何控制的心脏微组织促进血管化,减少炎症in vitro和in vivo

Yimu Zhao^1,2,3,4, Ramak Khosravi^2,5,4, Krisco Cheung^6,4

¹Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3E3, Canada.

Cell biomaterials

|August 7, 2025

概括

几何控制的心脏微组织改善血管化和移植,用于心脏修复. 与分散的细胞相比,这些工程组织减少了炎症和细胞死亡,提供了一个有希望的再生策略.

科学领域:

生物医学工程生物医学工程
再生医学是一种再生医学.
心血管研究研究心血管研究

背景情况:

心脏组织工程面临的挑战包括不良的血管化,移植和炎症控制.
目前的策略往往无法为工程心脏组织提供足够的支持.

研究的目的:

研究几何控制心脏微组织对心脏修复的益处.
为了比较微组织与分散细胞在促进移植,血管化和减少炎症方面的疗效.

主要方法:

人类诱导的多能干细胞衍生心肌细胞 (hiPSC-CMs) 用于创建在聚甲基西洛 (PDMS) 支柱之间附着的微组织.
微组织植入到裸体大鼠的预兆中.
评估了细胞对齐,收缩功能,移植,血管化,炎症标志物,细胞死亡 (LDH),细胞毒性 (mtDNA),YAP激活和细胞因子概况.

主要成果:

微组织表现出强大的植入,收缩性和血管化,植入后炎症显著减少.
与分散的细胞相比,微组织显示出增强的血管网络形成,减少细胞死亡和细胞毒性.
微组织表现出改变的YAP激活和有利的细胞因子配置,具有高的亲血管原因子和减少的炎症标志物.

结论:

几何控制的心脏微组织作为心脏组织工程的有效基石.
微组织植入促进了增强的血管化,移植和减少炎症,为心脏修复和再生提供了一个有希望的方法.

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