相关实验视频

Updated: Sep 12, 2025

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Published on: September 23, 2021

乳腺癌微环境的免疫调节由瘤衍生的外体:对免疫疗法的影响

Sepideh Maralbashi^1,2, Cynthia Aslan^3,4, Houman Kahroba^5,6

¹Drug applied research center, Tabriz University of Medical Sciences, Tabriz, Iran.

Cancer investigation

|August 7, 2025

概括

乳腺癌衍生的外体细胞 (BEXs) 通过提供免疫抑制分子,如CD39,CD73和PD-L1.1,来抑制免疫反应. 这些外体在免疫逃避中起着关键作用,是癌症免疫治疗的潜在目标.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

外体是细胞分泌的纳米级细胞外囊泡,包括癌细胞.
来自癌症的外体细胞调解细胞间通信,影响瘤的进展,血管新生,转移和免疫逃避.
乳腺癌衍生的外体细胞 (BEXs) 参与调节瘤微环境和免疫反应.

研究的目的:

阐明乳腺癌衍生的外体细胞所使用的免疫调节机制.
研究BEX上特定分子在免疫抑制和免疫检查点相互作用中的作用.
突出BEXs作为乳腺癌免疫治疗中的诊断和治疗点的潜力.

主要方法:

乳腺癌衍生的外体内分子载荷的表征.
通过BEXs对ectoenzyme活性 (CD39,CD73) 和腺生成的分析.
评估BEXs上的免疫检查点分子表达 (PD-L1,CD200,CD47) 以及它们与免疫细胞的相互作用.

主要成果:

贝克斯表达免疫调节性赤外酶CD39和CD73,它们从ATP产生腺,促进免疫抑制.
贝克斯携带免疫检查点连接体,包括编程死亡连接体1 (PD-L1),CD200和CD47,它们与PD-1,CD200R和SIRPα相互作用,分别抑制免疫监测.
发现编程死亡连接体1 (PD-L1) 在外体细胞上比在瘤细胞表面更为丰富,强调外体细胞介导的免疫逃避.

结论:

乳腺癌衍生的外基因组通过分子载荷传递积极抑制抗瘤免疫力.
在BEXs上的免疫抑制酶和免疫检查点连接体的表达突出了它们在促进癌症免疫逃避中的关键作用.
贝克斯代表了有前途的生物标志物用于诊断和潜在的治疗目标,以提高乳腺癌免疫疗法的疗效.

关键词:

乳腺癌是什么? 乳腺癌是什么 CD39 CD39 是一个 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 在PD-L1中.来自瘤的外体细胞.

更多相关视频

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...