20E诱导的Kr-h1表达促进了根据BR-C增强剂的染色体可访问性的发展过渡
Shihui Long1, Yongyu Qiu1, Tong Lin1
1Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
这项研究揭示了Drosophila中20-hydroxyecdysone (20E) 激活的Kr-h1-BR-C通路是如何在幼虫-prepupal过渡期间对翼盘发育至关重要. 这涉及荷尔蒙信号传递,转录因子和基因组修饰,以调节昆虫转型的基因表达.
科学领域:
- 发展生物学 发展生物学
- 分子内分泌学分子内分泌学
- 昆虫遗传学 昆虫遗传学
背景情况:
- 基因表达受到转录因子和影响染色质可访问性的基因基因修饰的严格调节.
- 在整体代谢昆虫中,青年激素激活的Kr-h1通常会抵消20-基 (20E) 诱导的幼虫特异体*BR-C*的表达,保持幼虫状态.
研究的目的:
- 为了阐明20E激活的Kr-h1-BR-C轴在Drosophila melanogaster*幼虫-prepupal过渡期间的翼盘形态发生过程中的作用.
- 研究20E信号调节*Kr-h1*和*BR-C*表达和染色质可访问性的分子机制.
主要方法:
- 在幼虫-prepupal过渡期间对基因表达模式的分析.
- 在*BR-C*增强剂中对转录因子结合和基因素修饰 (H3K27乙化) 的研究.
- 使用Drosophila melanogaster作为一个模型生物.
主要成果:
- 激活20E的*Kr-h1-BR-C*轴对翼盘发育至关重要.
- 20E信号直接激活Kr-h1,然后通过*BR-C*增强器中的正Kr-h1结合位点 (PKBS) 上调*BR-C*表达.
- 20E诱导的H3K27乙化增强了PKBS含有的增强剂的染色质可访问性,最大限度地提高了发育过渡的*BR-C*表达.
结论:
- 一个单一的转录因子可以根据激素刺激和不同增强器区域的染色质可访问性来差异调节相同的基因的表达.
- 这种机制允许通过上下文依赖的基因调节来操纵不同的发育事件,例如变形.
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