克里斯普尔-卡斯9查揭示了调节脂肪细胞增殖和脂质代谢的微蛋白
Victor J Pai1, Huanqi Shan1, Cynthia J Donaldson1
1Clayton Foundation Peptide Biology Laboratories, The Salk Institute for Biological Studies, La Jolla, CA 92037.
概括
研究人员确定了由小型开放式读取 (smORF) 编码的新型微蛋白,该微蛋白调节脂肪细胞 (脂肪细胞) 增殖和分化. 这项研究利用CRISPR查揭示了代谢过程中的这些关键调节者.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 代谢研究研究 代谢研究
背景情况:
- 小开放式读取框架 (smORF) 编码涉及生物过程的微蛋白.
- 在脂肪细胞生物学中smORFs的功能在很大程度上是未知的.
- 以前的研究已经在小鼠脂肪细胞中发现了数千个smORF.
研究的目的:
- 通过3T3-L1前脂肪细胞模型在脂肪细胞中识别新型smORF.
- 研究smORFs在脂肪细胞增殖和分化中的功能性作用.
- 探索微蛋白在代谢调节中的潜力.
主要方法:
- 核糖体简介用于识别smORFs.
- 在CRISPR/Cas9单向导RNA (sgRNA) 库中进行选.
- 脱落屏幕和光辅助的细胞分拣脂质滴.
主要成果:
- 确定了许多调节脂肪细胞增殖和脂质积累的smORF.
- 发现了smORFs在cis调节作用 (uORFs,dORFs) 和功能性微蛋白中发挥作用.
- 证明了一种来自lncRNA 923011K14Rik的特定微蛋白调节脂肪细胞分化.
结论:
- 基于CRISPR的查对于发现功能性smORFs是有效的.
- 由smORFs编码的微蛋白是脂肪细胞生物学的重要调节者.
- 这项工作为研究微蛋白在代谢健康方面提供了基础.
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