氧化糖发酵过程中的乙酸代谢增强了工程酸耐受性Issatchenkia orientalis中的3基酸生产
Deokyeol Jeong1, Dahye Lee1, Junli Liu2
1Department of Food Science, Purdue University, West Lafayette, IN 47907, USA; Whistler Center for Carbohydrate Research, Purdue University, West Lafayette, IN 47907, USA.
Bioresource technology
|August 7, 2025
概括
在这项研究中,从富含乙酸的生物质中改造了酵母,以产生3-基酸 (3-HP). 这种耐酸菌株获得了高的3HP标位,证明了生物塑料前体生产的潜力.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 林氏纤维素生物质中的酸抑制了生物转化.
- 有效地生产生物塑料前体3基酸 (3-HP) 是一个挑战.
- 东方酵母是一种非传统的酵母,具有工业应用的潜力.
研究的目的:
- 为了设计一种耐酸的Issatchenkia orientalis菌株,从葡萄糖,糖和酸盐中产生3-HP.
- 为了克服酸毒性在林氏纤维素生物质生物转化中.
- 为了优化使用工程酵母的3HP生产.
主要方法:
- 开发了一个耐酸的Issatchenkia orientalis菌株 (IoDY01H).
- 利用基于Cas9的基因组编辑来引入基利用和基于β-氨酸的3-HP途径.
- 进行了代谢分析,以了解代谢流的重定向.
- 使用预处理的麻茎水解剂进行发酵实验.
主要成果:
- 经过工程设计的IoDY01H从葡萄糖,糖和酸盐中产生3-HP.
- 乙补充剂增强了3-HP的产生,但导致β-氨酸的积累.
- 在pH 5.5的发酵中,比pH 3.5.的发酵产生更高的3HP标位.
- 通过分离水解和发酵 (SHF) 使用大麻茎水解剂达到8.7g/L的3HP标位.
结论:
- 通过使用工程酵母,证明了从富含乙酸盐的生物质中生产3-HP的可行性.
- 突出了Issatchenkia orientalis作为一个有前途的微生物底盘,用于工业生物转化.
- 在酸性条件下确定了β-氨酸-酸盐氨基转移酶活性的潜在限制.
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