解码关键的CAZyme基因和转录因子,用于通过发酵抑制病原体的菌纤维素生物质的价值化
Peng Ren1, Tianjie Yang1, Xinlan Mei1
1Key Lab of Organic-based Fertilizers of China, Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Weigang 1, Nanjing 210095, PR China.
Bioresource technology
|August 7, 2025
概括
桃茎的Bacillus amyloliquefaciens发酵产生了强大的病原体抑制剂. 通过RpoE调节的关键葡萄糖转移酶和多糖酶基因被确定用于生物价值化.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 碳水化合物活性酶 (CAZyme) 基因和转录因子 (TFs) 对于纤维素生物质发酵和病原体抑制至关重要.
- 识别CAZyme多样性和TF复杂性阻碍了生物资源的有效生物价值化.
研究的目的:
- 为了抑制病原体Ralstonia solanacearum使用Bacillus amyloliquefaciens发酵香和花生茎.
- 用多omics和机器学习分析关键的CAZyme基因及其TFs.
主要方法:
- 用Bacillus amyloliquefaciens发酵花莲和花生茎.
- 多组学分析 (基因组学,转录组学) 和用于基因和TF识别的机器学习.
- 发酵提取物的抗菌活性评估.
主要成果:
- 发酵花茎 (6-7天) 的水溶性提取物显示出强烈的抗菌活性,对抗Ralstonia solanacearum.
- 在花茎发酵过程中显著丰富了糖转移酶 (GT) 和多糖酶 (PL) 基因组.
- 鉴定UDP-glycosyltransferase (GT1) 和pectin lyase (PL1) 基因作为抑制的关键贡献者,RpoE被预测为PL1.1的积极调节者.
结论:
- 这项研究成功地确定了关键的CAZyme基因及其参与生物质发酵期间病原体抑制的TFs.
- 为快速发现TF和优化生物资源价值化发酵技术提供了基础.
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