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亚姆多糖化物通过有针对性的CASP3激活抑制促进了MC3T3-E1细胞的骨分化

Qiting Yu¹, LinHui Liu², TaiPing Jiang³

¹Guizhou University of Traditional Chinese Medicine, Guiyang, 550000, Guizhou, China.

Journal of orthopaedic surgery and research

|August 7, 2025

概括

雅姆多糖 (YP) 通过促进骨细胞生长和分化,在治疗骨质疏松症方面表现有前途. 它通过调节CASP3表达和AKT/PI3K信号通路来起作用,为骨健康提供了潜在的天然治疗方法.

科学领域:

药理学药理学是指药理学的学科.
生物化学生物化学
细胞生物学细胞生物学

背景情况:

骨质疏松症 (OP) 是一种骨代谢障碍,导致骨质减少和骨折风险增加.
雅姆多糖 (YP),来自Dioscorea opposita,已知具有抗炎和抗氧化作用,但其在骨分化中的作用尚不清楚.

研究的目的:

为了研究多糖 (YP) 在骨质疏松症 (OP) 中的保护机制.

主要方法:

网络药理学确定了核心目标 (CASP3,HMGCR,HSP90AA1,PTGS2) 和途径.
分子对接预测了YP与目标的结合亲和力.
在体外研究中使用MC3T3-E1细胞通过细胞活力,ALP,Alizarin红色染色和qPCR来评估YP的骨质生成潜力.

主要成果:

YP表现出与核心目标的良好结合,并与81个与OP相关的通路相互作用,特别是PPAR信号通路.
在体外,YP显著促进了MC3T3-E1细胞增殖,增强了性酸酶活性,增加了矿化结节的形成,并提高了骨质生殖标志物.
在200ug/mL YP下观察到最佳效果,与CASP3调节和AKT/PI3K通路有关.

结论:

网络药理学确定了CASP3和AKT/PI3K途径是YP在OP中的作用的关键调解者.
YP显示出作为骨质疏松症的天然抑制剂的潜力,特别是当过度的CASP3激活驱动时.

关键词:

在MC3T3-E1细胞中.骨质母细胞是骨质母细胞.骨质疏松症是一种骨质疏松症.亚姆的多糖是亚姆的多糖.

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