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MADS盒子基因CmAP1促进了中国栗子 (Castanea mollissima) 的开花和花发育

Yunhe Cheng^1,2, Lili Cheng^1,2, Guanglong Hu^1,2

¹Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100093, China.

BMC plant biology

|August 8, 2025

概括

中国栗子APETALA 1 (AP1) 基因CmAP1促进早期开花,对花发育至关重要. 这项研究提供了对中国栗子花期调节的见解,有助于提高产量.

科学领域:

植物分子生物学植物分子生物学
植物遗传学植物遗传学
发展生物学发展生物学

背景情况:

中国栗子 (Castanea mollissima) 是一种重要的坚果作物,其开花直接影响产量.
众所周知,MADS盒子基因APETALA 1 (AP1) 在各种植物物种的花启动和发育中发挥着重要作用.
在中国栗子中CmAP1基因的特定功能在很大程度上仍未被描述.

研究的目的:

为了克隆和表征APETALA 1 (AP1) 基因,命名为CmAP1,来自中国栗子 (Castanea mollissima).
研究CmAP1在花发育,发育,特别是中国栗子花形成中的功能性作用.

主要方法:

克隆编码序列 (CDS) 和CmAP1基因的促进器.
亚细胞局部化测试以确定CmAP1蛋白位址.
GUS记者在转基因Arabidopsis thaliana中进行了测试,以分析促进体活性.
现场RNA杂交以检查花朵发育期间的CmAP1表达模式.
在Arabidopsis突变体中CmAP1的异质表达,以评估其在开花和花器官发育中的功能.

主要成果:

CmAP1基因编码了一种246氨基酸蛋白质,该蛋白质定位到核中.
检测到CmAP1的表达在各种花的meristems和原始,与其促进器活跃在苗木和植物器官的转基因Arabidopsis.
对CmAP1促进体的分析揭示了潜在的调节元素,包括奥克辛和莉酸反应部位.
在Arabidopsis中,CmAP1的异质表达导致了加速的开花,并挽救了ap1-11突变体中的花旋缺陷,并上调了花 меристем身份基因.

结论:

在中国栗子中,CmAP1积极调节开花时间,并在花器官,特别是花的发育中发挥关键作用.
了解CmAP1的功能为分子育种策略提供了基础,以提高中国栗子产量.
这项研究阐明了Castanea mollissima中开花和花发育调节的关键方面.

关键词:

一个APETALA1一个APETALA.卡斯塔尼亚海 (Castanea mollissima) 是一个很小的鱼类.花的器官发育植物器官发育开花的开花时间基因表达基因表达基因表达

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