相关实验视频

Updated: Sep 12, 2025

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Published on: June 17, 2022

在HL60急性骨髓性白血病细胞中,特定的microRNA表达和辐射抵抗条件之间的关系

Hikoto Sugiyama¹, Megumi Kikuchi¹, Mitsuru Chiba²

¹Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan.

Molecular medicine reports

|August 8, 2025

概括

研究人员在耐辐射急性髓性白血病 (AML) 细胞中发现了五种具有改变表达的微RNA (miRNA). 这些miRNAs可以通过调节参与细胞周期和细胞亡的基因来影响AML的放射电阻.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
遗传学遗传学是一个

背景情况:

急性髓性白血病 (AML) 治疗通常涉及高剂量辐射,但可以出现耐辐射细胞,阻碍治疗.
微RNA (miRNA) 表达在AML放射电阻的发展中的作用尚不清楚.

研究的目的:

在抗辐射AML细胞模型中研究miRNA表达特征.
为了确定特定的miRNA及其潜在的基因参与AML放射电阻.

主要方法:

在暴露于辐射的HL60细胞系 (Res-HL60) 上利用miRNA微阵列分析,与野生型HL60细胞相比.
使用逆转录定量PCR验证了关键miRNAs的表达水平.
采用OmicsNet和Reactome路径分析来识别目标mRNA和相关的生物过程.

主要成果:

在Res-HL60和野生型HL60细胞之间鉴定了27个差异表达的miRNA (10个上调,17个下调).
验证了五种特定的miRNAs (miR-146a-5p上调;miR-30c-1-3p,miR-671-5p,miR-610,miR-3675-5p下调).
确定了27个向mRNA,并揭示了"细胞周期,线粒细胞","细胞亡"和"免疫系统"途径的丰富.

结论:

五种特定的miRNAs的改变表达与抗辐射AML有关.
这些miRNAs可能通过调节关键细胞通路中的mRNA表达来调节AML放射电阻.

关键词:

在DNA碎片中,有DNA碎片.在 HL6060 中,生物信息学是一种生物信息学.这种白血病是白血病.这是一个微型RNA.具有无线电阻抗性的

更多相关视频

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...