相关实验视频

Updated: Sep 12, 2025

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Published on: April 26, 2019

联合删除miR-27a和miR-27b可增强对饮食引起的肥胖的保护

Devesh Kesharwani¹, Michele Karolak¹, Chad C Doucette¹

¹Center for Molecular Medicine, MaineHealth Institute for Research, 81 Research Drive, Scarborough, ME 04074.

bioRxiv : the preprint server for biology

|August 8, 2025

概括

微RNAs miR-27a 和 miR-27b 抑制脂肪组织的热生成,恶化肥胖. 删除既增强了热生成,也对抗了代谢功能障碍,提供了一个潜在的治疗点.

科学领域:

代谢研究的研究.
肥胖研究的研究.
脂肪组织生物学脂肪组织生物学

背景情况:

肥胖与脂肪组织热生成受损和代谢功能障碍有关.
在小鼠中,饮食诱导的肥胖 (DIO) 抑制了白色脂肪组织 (WAT) 中的关键发热基因.
在DIO期间,miR-27a和miR-27b表达增加了WAT.

研究的目的:

研究miR-27a和miR-27b在调节脂肪组织热生成和脂肪生成编程中的作用.
确定是否针对miR-27a/b可以改善饮食引起的肥胖和代谢功能障碍.

主要方法:

使用全球淘汰赛小鼠模型用于miR-27a和miR-27b.
向野生型,单击淘汰和双击淘汰 (DKO) 的小鼠进行高脂肪饮食 (HFD).
对脂肪细胞进行了体外和体外分析.
测量了基因表达 (Ucp1,Ppary,Pgc1a),线粒体热量产生,WAT棕色化,葡萄糖和胰岛素敏感性和脂肪性.

主要成果:

联合删除miR-27a和miR-27b (DKO) 协同增强了Ucp1表达,线粒体热量产生和WAT色.
与单次淘汰赛相比,DKO小鼠在HFD条件下表现出改善的葡萄糖和胰岛素敏感性和减少的脂肪性.
在体外和体外研究证实了DKO脂肪细胞中发热基因表达的增加和脂质积累的减少.

结论:

miR-27a和miR-27b共同抑制脂肪热生成,并促进肥胖的代谢功能障碍.
针对miR-27a/b轴提出了一种新的治疗策略,用于提高能源消耗和打击与肥胖相关的代谢疾病.

关键词:

黄色的脂肪细胞生物科学生物科学生理学生理学生理学在 miR-27a 中.这就是miR-27b.肥胖肥胖肥胖肥胖肥胖肥胖肥胖肥胖皮下脂肪组织的脂肪组织.热生成是一种热生成.

更多相关视频

Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice

Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice

Published on: October 18, 2024

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum Clove and Cuminun cyminum Cumin on C57BL6/j Mice Fed High-Fat Diet

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum Clove and Cuminun cyminum Cumin on C57BL6/j Mice Fed High-Fat Diet

Published on: July 31, 2021

相关概念视频

Obesity

Obesity

The Body Mass Index (BMI) is a numerical value derived from a person's weight and height, used to categorize individuals into weight ranges. It is calculated using the formula: weight in kilograms divided by height in meters squared. Obesity is a health condition characterized by excessive accumulation of adipose tissue that poses health risks, often diagnosed with a BMI ≥ 30. This excess fat storage occurs when surplus dietary calories are converted into triglycerides and stored in...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Regulation of Food Intake

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...