在Streptococcus mutans中通过氨酸/氨酸酸化和GpsB进行翻译后修饰
Sangam Chudal1, Courtney Dover1, Tiffany Haydt1,2
1Department of Biological Sciences, Beck College of Sciences and Mathematics, Arkansas State University, Jonesboro, Arkansas, USA.
bioRxiv : the preprint server for biology
|August 8, 2025
概括
这项研究揭示了Streptococcus mutans中广泛的O-酸化,影响了翻译和新陈代谢等核心功能. 关键调节者PknB和PPPL控制着不同的酸化标,影响细菌生理学.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 翻译后修饰 (PTMs),特别是蛋白质酸化,对于调节细菌生理学至关重要.
- 突变性链球菌依赖于酸化来进行必要的细胞过程.
研究的目的:
- 在非压力条件下对Streptococcus mutans进行首次全面的蛋白组分析.
- 为了研究蛋白质酸化调节中蛋白质激酶 (PknB) 和酸酶 (PppL) 的作用.
- 识别由PknB和PppL控制的基板和信号通路.
主要方法:
- 基于Tandem质标记 (TMT) 的质谱法用于蛋白质组分析.
- 用聚丰富技术来识别化部位.
- 在S. mutans的野生类型和淘汰突变 (ΔpknB, ΔpppL) 上进行了蛋白质和蛋白质分析.
主要成果:
- 在131种蛋白质上鉴定出231种高保证度酸,表明O-酸化广泛存在.
- 酸化蛋白质参与翻译,碳水化合物代谢和细胞循环调节.
- 对ΔpknB和ΔpppL突变的分析揭示了不同的激酶和酸酶基质,包括DIVIVA,MapZ,MltG和核糖体蛋白质.
- 发现GpsB抑制导致致命的缺陷被PPPL抑制器突变所挽救,恢复DIVIVA酸化并突出显示PknB/PppL信号轴.
结论:
- 在Streptococcus mutans中,O-酸化在调节细胞核心功能方面发挥着广泛的作用.
- PknB和PppL是细菌光信号的关键调节者,具有重叠但独特的基质特异性.
- 可能由GpsB调节的PknB/PppL信号轴对于维持S. mutans关键目标的酸化至关重要.
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