相关实验视频

Updated: Sep 12, 2025

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Published on: May 16, 2021

在小鼠胚胎塞尔托利细胞中的糖原代谢维持了通过乳酸穿的生殖系

Martín A Estermann^1,2, Joseph Sheheen^3,2, Sara A Grimm⁴

¹Reproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.

bioRxiv : the preprint server for biology

|August 8, 2025

概括

男子胚胎丸依靠塞尔托利细胞中的糖原来发育生殖细胞. 破坏这种途径会损害生殖细胞的殖民,从而揭示出生育的关键代谢窗口.

科学领域:

生殖生物学生殖生物学
发育生物学是发展生物学.
代谢过程中的代谢.

背景情况:

代谢物调节细胞命运和血统承诺.
丸分化的遗传途径已知,但代谢作用尚不清楚.
塞尔托利细胞对于丸发育至关重要.

研究的目的:

研究新陈代谢在胚胎丸发育中的作用.
检查Sertoli细胞中过渡性糖原积累的功能.
了解塞尔托利和生殖细胞之间的代谢合.

主要方法:

在小鼠胚胎塞尔托利细胞中研究了糖原代谢.
干扰糖原代谢和抑制乳酸运输.
评估了生殖细胞的殖民化和代谢合.

主要成果:

糖原代谢对于塞尔托利细胞分化是不可或缺的,但对于胚胎细胞殖民至关重要.
干扰减少了乳酸盐的产生,并影响了生殖细胞的迁移.
确定了Sertoli和生殖细胞之间的代谢合.
外部乳酸或葡萄糖补充剂并没有拯救生殖细胞表型.

结论:

塞尔托利细胞的糖原积累支持早期丸发育中的关键代谢窗口.
胚胎细胞在代谢上受到限制,在这个窗口期间不能利用外部碳来源.
在此期间缺乏营养素可能会影响长期的生育能力.

关键词:

生育能力生育能力生育能力乳酸航天飞机乳酸飞机代谢合的代谢合代谢过程中的代谢.性别的确定性性别的确定性精子精子精子

更多相关视频

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Published on: September 17, 2019

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

相关概念视频

Spermatogenesis

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...

Glycolysis

Glycolysis

Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...