Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Inhibitors of Viral Protein Synthesis

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

HIV-1 capsid interactions with Nuclear Pore Complex components support nuclear entry via affinity gradient.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Structural and Mechanistic Basis of F227C-Mediated Hypersusceptibility to Islatravir in HIV-1 Reverse Transcriptase.

bioRxiv : the preprint server for biology·2026

Same author

Unraveling the Mechanism of HIV-1 Hypersusceptibility to Tenofovir Imparted by Islatravir Resistance Mutations.

bioRxiv : the preprint server for biology·2026

Same author

Catching our breath: development of interventions and therapies for respiratory syncytial virus.

Microbiology and molecular biology reviews : MMBR·2026

Same author

Structural Basis of Polypurine Track Strand Displacement by HIV-1 Reverse Transcriptase.

bioRxiv : the preprint server for biology·2026

Same author

Damaging the conical morphology of HIV-1 capsid by targeting the FG-binding pocket and disfavoring pentameric subunits needed for core closure.

bioRxiv : the preprint server for biology·2026

Search research articles

相关实验视频

Updated: Jun 13, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

使用生物层干涉测量的抗病毒化合物的表征.

Zachary C Lorson^1,2, William M McFadden^1,2, Grace Neilsen^1,2

¹Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, 1760 Haygood Drive NE, Atlanta, GA.

bioRxiv : the preprint server for biology

|August 8, 2025

概括

优化的生物层干扰测量 (BLI) 协议有效地描述了用于抗病毒发现的小分子-蛋白相互作用. 这种高通量方法克服了常见的陷,使药物开发的精确亲和度测量成为可能.

关键词:

生物层干涉测量 (BLI) 是一种生物层干涉测量.生物分子相互作用卡普西德蛋白 (CA) 是一种人类免疫缺陷病毒 (HIV)莱纳卡帕维尔 (LEN) 是一种主要蛋白质酶nsp5 尼尔马特雷尔维尔 (NIR) 是一种药物.严重急性呼吸系统综合征冠状病毒-2 (SARS-CoV-2)

更多相关视频

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

相关实验视频

Last Updated: Jun 13, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

科学领域:

生物化学和分子生物学
药物发现和开发药物发现和开发
生物物理化学生物物理化学

背景情况:

小分子-蛋白相互作用对于生物功能和疾病治疗至关重要.
生物层干涉测量 (BLI) 为研究这些相互作用提供了一种敏感,高通量的方法,特别是在抗病毒发现中.
诸如非特异性结合和带漂移等挑战可能会阻碍BLI的准确性.

研究的目的:

为描述小分子-蛋白相互作用提供优化的BLI协议.
解决和提供解决BLI常见陷的问题.
为了证明BLI在计算临床相关抗病毒化合物的结合 afinities 的实用性.

主要方法:

开发和应用优化的BLI协议.
描述PF74和HIV-1囊蛋白 (CA) 之间的相互作用.
对Lenacapavir (LEN) 与HIV-1 CA和Nirmatrelvir (NIR) 与SARS-CoV-2 Mpro.的分析

主要成果:

证明BLI是计算从微到亚纳米级范围的结合亲和力 (K_D值) 的强大工具.
对HIV-1 CA与PF74和LEN的相互作用的量化结合亲缘关系.
证实了尼尔马特雷尔维尔和SARS-CoV-2 Mpro.之间的共价相互作用.

结论:

优化的BLI协议促进了小分子与蛋白质结合的准确表征.
BLI有效地确定了抗病毒药物候选者的结合 afinities 和相互作用类型.
这些协议支持新型抗病毒药物和衍生品的高通量查和测试.