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Updated: Sep 12, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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没有序列限制的CRISPR/Cas系统引导的等离子体突变发生

Fengjiao Zhao1, Feng Chen1, Huahang Yu1

  • 1Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

Fundamental research
|August 8, 2025
PubMed
概括

这项研究介绍了CRISPR/Cas系统引导的等离子体突变发生,用于工程蛋白质变体. 新方法有效地在等离子体上创建用户定义的突变库,改进蛋白质工程和基因组编辑工具.

关键词:
这是CRISPR/Cas系统.DNA组装组件的组装等离子体突变发生在等离子体中.蛋白质工程是一种蛋白质工程.序列可编程性 序列可编程性

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