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一个基于aptamer的电化学传感器用于检测牛奶中的氨基糖化物:解决实际挑战

Emeka J Itumoh¹, Yasmin Liu¹, Erica Cassie¹

¹Forensic Research and Development Department, New Zealand Institute for Public Health and Forensic Science (PHF Science), Kenepuru Science Centre: 34 Kenepuru Drive, Kenepuru, Porirua 5022 PO Box 50348, Porirua 5240, New Zealand. eitu709@aucklanduni.ac.nz.

|August 8, 2025

概括

这项研究开发了一种基于aptamer的实用电化学传感器,用于检测牛奶中的氨基糖化物. 优化的制造和简单的样品稀释使得可靠的现场检测能够提高灵敏度.

科学领域:

生物传感器和电化学传感器
食品安全和质量控制
分析化学是一种分析化学.

背景情况:

氨基糖化物是畜牧业中至关重要的抗生素,需要在牛奶中使用可靠的检测方法.
基于电化学体 (E-AB) 的传感器为敏感和特定的分析物检测提供了一个有前途的平台.
优化传感器性能和保质期对于实际的现场应用至关重要.

研究的目的:

研究和优化E-AB传感器用于乳中的氨基糖化物检测.
评估影响传感器性能的因素,包括电极材料,清洁和样品准备.
评估在牛奶中现场检测氨基糖化物的可行性.

主要方法:

在激光剥离电极 (PET基板) 和印电极 (陶基板) 上制造E-AB传感器.
电化学清洁方案的评估 (H2SO4潜在循环与KOH/H2O2氧化).
牛奶样本预处理方法 (SPE,MeCN,酸,卡雷兹) 和简单稀释 (PBS缓冲) 的比较.
在各种储存条件下 (4°C水中) 评估传感器的保质期.
现场检测的演示,使用微型电压器和手机.

主要成果:

与丝网打印电极相比,PET基板上的激光剥离电极在检测极限 (LOD) 中显示了超过84%的改进.
使用H2SO4电位循环的电化学清洗优于氧化清洗.
简单的乳液稀释 (50%在PBS缓冲器中) 被确定为现场检测的合适方法,产生100μM的LOD.
当传感器在4°C的水中储存时,传感器在5天内保持稳定的性能.
通过便携式系统成功演示了现场检测.

结论:

在激光切除的PET电极上制造的优化E-AB传感器为牛奶中氨基糖化物检测提供了更高的性能.
简单的乳液稀释是现场传感应用中样品制备的实用方法.
开发的传感器系统显示出快速,灵敏和现场监测牛奶中的氨基甘油酸的巨大潜力,有助于提高食品安全.

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