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相关概念视频

Synthetic Biology02:55

Synthetic Biology

5.0K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
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走向一个单一的SynBio工具包:评估监管要素的性能和eudicot兼容性.

David B May1, Alexander C Pfotenhauer1, Bryn L Concha1

  • 1Center for Agricultural Synthetic Biology, University of Tennessee Institute of Agriculture, Knoxville, TN, 37996, USA.

Plant cell reports
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概括

研究人员开发了一套合成生物学工具包,其中包含遗传调节元素,用于改善玉米等单种植物中的转基因表达. 该图书馆为农业和基因工程中的应用提供了广泛的动态范围.

关键词:
测仪的光测量方法遗传调节元素的遗传调节元素它们是单角鸟类.原始塑料是原始塑料中的一个.合成生物学 合成生物学转基因表达的表达方式

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科学领域:

  • 合成生物学 合成生物学
  • 植物分子生物学 植物分子生物学
  • 基因工程是一种基因工程.

背景情况:

  • 单类物种对全球农业至关重要,需要在基因工程方面取得进展.
  • 适合的遗传调节元素的有限可用性阻碍了合成生物学在谷物和草的应用.

研究的目的:

  • 组装和表征一个全面的工具包,用于单种植物的遗传调节元素.
  • 为了使可调节的转基因表达在农业物种中用于合成生物学应用.

主要方法:

  • 开发了一个由80个成员组成的组合部分库,包含来自不同来源的发起者,5' UTR 和 3' UTR.
  • 在Zea mays原生体中评估了77种GFP记者基因表达的组合.
  • 通过农业透,评估了Nicotiana benthamiana中的监管元素的交叉合性.

主要成果:

  • 在Zea mays原生体中达到74.5倍的GFP表达水平的动态范围.
  • 确定了新型促进物-5' UTR 和 3' UTR 组合,可增强记者基因表达.
  • 在单和双物种的34种调节元素组合中表现出跨物种活性.

结论:

  • 开发的工具包为增强转基因表达在单虫中提供了宝贵的资源.
  • 这个工具包有助于各种合成生物学应用,包括基因组编辑和代谢途径工程.
  • 具有特征的元素提供了可调节的表达,这对于农作物中先进的基因操纵至关重要.