试管体内肌肉收缩:C2C12细胞中电脉冲刺激的技术综述
Mark R C van de Meene1, Anita M van den Hoek2, Roeland Hanemaaijer2
1Department of Microbiology and Systems Biology, The Netherlands Organization for Applied Scientific Research (TNO), Leiden, The Netherlands.
Experimental physiology
|August 8, 2025
概括
在C2C12细胞模型中的电脉冲刺激 (EPS) 在体外进行. 本综述分析了常见的EPS协议,突出了脉冲持续时间和电压等参数的变化,并建议标准化以提高研究可重复性.
科学领域:
- 肌肉生理学和细胞生物学
- 生物医学工程和运动科学
背景情况:
- 电脉冲刺激 (EPS) 对于体外骨肌肉运动建模至关重要.
- 在使用C2C12神经管的研究中,EPS协议的变化阻碍了可重现性.
- 标准化EPS参数对于可靠和可比的研究成果至关重要.
研究的目的:
- 在C2C12神经管研究中审查和分析常见的电脉冲刺激 (EPS) 协议.
- 为了识别经常使用的EPS参数 (脉冲持续时间,频率,电压,刺激持续时间).
- 在C2C12模型中使用EPS研究的生物过程的总结.
主要方法:
- 在C2C12细胞中使用EPS的54项同行评审研究的系统综述.
- 提取和分析EPS协议参数.
- 生物终点和研究主题的总结.
主要成果:
- 大多数研究使用了2毫秒的脉冲1赫兹和10-20V超过24小时.
- 葡萄糖吸收,AMPK激活,炎症和线粒体适应是常见的研究终点.
- 观察到脉冲持续时间,电压和刺激持续时间之间的相互依赖,频率更加标准化.
结论:
- 在EPS电压和脉冲持续时间中存在显著的变化,影响实验结果.
- 标准化报告和有意识的协议设计是需要的翻译相关性.
- 这一审查为选择特定研究目标的适当EPS参数提供了一个资源.
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