相关实验视频
Updated: Sep 12, 2025

13:07
One-channel Cell-attached Patch-clamp Recording
Published on: June 9, 2014
24.6K
氧化调节Piezo1通道的氧化调节
N Novosolova1, N Braidotti2, T Patinen1
1A. I. Virtanen Institute for Molecular Sciences, Faculty of Health Sciences, University of Eastern Finland, Kuopio, 70210, Finland.
Redox biology
|August 8, 2025
概括
氧化应激通过氧化关键氨基酸来抑制Piezo1通道活性,从而影响疾病中的细胞力学. 这种Piezo1通道的氧化还原调节在涉及氧化应激的条件下至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 神经科学是一个神经科学.
背景情况:
- 机械敏感的Piezo1通道与各种疾病病理有关.
- 氧化应激,以反应性氧物种 (ROS) 为特征,在神经退行性疾病中很常见.
- 皮埃佐1通道功能的氧化还原调节在很大程度上仍未被探索.
研究的目的:
- 研究氧化应激对Piezo1通道活性和透性的影响.
- 确定Piezo1通道激活是否影响内源性反应性氧物种 (ROS) 的产生.
- 阐明涉及Piezo1.1氧化还原调制的特定氨基酸残留物.
主要方法:
- 使用的红细胞 (RBC) 和表达Piezo1.3的HEK293T细胞.
- 采用了流细胞计,Ca2+成像,补丁和微吸技术.
- 使用了选择性氧化剂 (H2O2,Chloramine-T,DTNB) 和抗氧化剂 (N-乙囊,dithiothreitol),以及H2O2传感器HyPer7.
主要成果:
- 细胞透的氧化剂 (H2O2,胺-T) 和膜透的DTNB抑制了由Yoda1诱导的Piezo1激活.
- 过氧化降低了红细胞中Piezo1的机械敏感性.
- 抗氧化剂逆转了抑制作用,而Nrf2过度表达并没有阻止抑制,这表明作用部位受膜界限,其中氨酸发挥了主导作用.
结论:
- 氧化应激通过囊素和氨酸的氧化,抑制了Piezo1通道的活性.
- 皮埃佐1激活可以略微增加内源的H2O2产量.
- 在氧化应激条件下,Piezo1通道的氧化调节是疾病病理学的重要因素.
相关概念视频
Mechanically-gated Ion Channels
6.7K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.7K
Ion Channels
88.1K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
88.1K
G-Protein Gated Ion Channels
4.8K
GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
Sensory...
4.8K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.6K
Non-gated Ion Channels
7.1K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
7.1K

