相关实验视频

Updated: Sep 12, 2025

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Published on: April 2, 2015

选择一个不相互作用的缓冲器,以α-cyclodextrin含有溶液

Marlene Storm Andersen¹, Simon Løv Thomsen¹, Christian Schönbeck²

¹Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark.

International journal of pharmaceutics

|August 8, 2025

概括

缓冲区的选择显著影响循环德克斯 (CD) 药物配方. 一些碳酸缓冲剂干扰α-环氧德素 (α-CD) 复合,而其他缓冲剂的相互作用很小,影响药物的溶解性和稳定性.

科学领域:

制药科学制药科学
物理化学物理化学
药物运输药物运输药物运输

背景情况:

活性药物成分 (API) 的溶解性和稳定性差,挑战了药物配方.
循环脱素 (CDs) 通过包含复合物的形成来增强API的溶解性和稳定性.
缓冲区选择对于基于CD的配方的pH控制至关重要,但潜在的缓冲区-CD相互作用尚未完全理解.

研究的目的:

调查十二种药学相关的缓冲剂对α-cyclodextrin (α-CD) 与1,9-nonanediol的含有复杂化的影响.
为了确定缓冲结构和循环德克斯类型对复杂化常数和热力学参数的影响.

主要方法:

使用异热定位热量计 (ITC) 来量化复合常数和热力学参数.
该研究评估了在各种缓冲系统的存在下α-CD和1,9-nonanediol之间的相互作用.
进行的比较分析包括标准α-CD和基化α-cyclopodextrin (HP-α-CD).

主要成果:

缓冲物种显著影响了与1,9-nonanediol的α-CD复合.
碳酸缓冲剂 (烟酸,酸,酸,酸,酸) 显示出具有竞争力的结合,减少了复杂化.
酸盐,MES,Tris和酸显示出最小的干扰;碳酸酸上增加的基组降低了结合亲和力.

结论:

缓冲区的选择对于优化基于环氧德克斯的药物配方至关重要.
缓冲器-CD相互作用取决于缓冲器的化学结构和CD腔的特性.
HP-α-CD显示较低的结合常数,可能是由于硬质障碍和改变的腔体水友性.

关键词:

缓冲器缓冲器是一个缓冲器.复杂化常数是一个复杂化常数.异热定位热量计异热定位热量计它们的pH值为pH.α-Cyclodextrin 是一个环氧化物.

更多相关视频

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

相关概念视频

Buffer Effectiveness

Buffer Effectiveness

Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...

Ion Exchange

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...

Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...