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优化发酵条件,以提高Coriolus versicolor的乳糖生产,以及其降解2,4-二二醇的潜力

Daiyi Zheng^1,2, Jiafeng Li^1,2, Zhiyuan Yu^1,2

¹Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.

Bioprocess and biosystems engineering

|August 8, 2025

概括

这项研究优化了Coriolus versicolor的乳糖生产,以改善环境. 通过优化发酵和共同培养实现了增强的乳酶活性,从而有效降解了2,4-二二 (2,4-DCP).

科学领域:

环境科学环境科学
生物技术是生物技术.
生物催化剂是一种生物催化剂.

背景情况:

持久性有机污染物对环境构成重大挑战.
由于其氧化能力,乳糖是一种有前途的绿色生物催化剂,用于环境修复.
优化乳糖生产和应用对于有效降解污染物至关重要.

研究的目的:

为了优化Coriolus versicolor生产乳糖的条件.
调查食批量养和共同培养对乳糖分泌物的影响.
为了评估laccase对2,4-二二醇 (2,4-DCP) 的降解性能.

主要方法:

在Coriolus versicolor的浸泡发酵中,优化了小麦的度和pH值.
养批量养策略和与Penicillium共同种植,以提高乳糖生产.
2,4-DCP的乳酶介导降解试验,优化酶剂量,基质度和调解剂的添加.

主要成果:

在发酵的第六天,乳糖活性达到峰值;优化的小麦 (20 g/L) 和pH (5.0) 提高了产量.
食批量养和与Penicillium共同培养显著增加了乳糖分泌.
使用优化的乳糖酶条件和调解剂2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 实现了2,4-DCP的完全降解.

结论:

优化的发酵和共同种植策略有效地提高了Coriolus versicolor.的乳糖生产.
拉卡斯在降解持久性有机污染物2,4-DCP.中表现出高效率.
这项研究为在有机污染物的实际环境修复中使用laccase提供了基础.

关键词:

科里奥卢斯 (Coriolus versicolor) 是一种有色的植物.2,4-二二醇的使用方法生物降解生物降解双菌株发酵方式的发酵.拉卡斯 (Laccase) 是一个古老的城市.

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