多功能细胞工厂Aspergillus niger可以被设计成产生氧化原蛋白
Tom Morris1, Friederike Gerstl1, Sascha Jung1
1Chair of Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.
Biotechnology for biofuels and bioproducts
|August 9, 2025
概括
这项研究表明,Aspergillus niger可以产生氧化原蛋白,这是组织工程和伤口愈合的关键蛋白质. 这种微生物系统提供了一个无病毒的来源,以满足全球原蛋白需求.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 微生物学 微生物学
背景情况:
- 在组织打印和伤口愈合中,对原蛋白的全球需求正在增加.
- 微生物生产提供了一种无病毒的替代动物衍生的原蛋白.
- 亚斯伯吉拉斯尼格尔是一种丝状真菌,具有重组蛋白质生产的潜力,但尚未对原进行探索.
研究的目的:
- 为了设计Aspergillus niger用于生产氧化原蛋白.
- 为了优化发酵条件以提高原蛋白产量.
- 建立Aspergillus niger作为一种可行的微生物细胞工厂,用于无病毒的原蛋白.
主要方法:
- 基因工程使用模块化克隆引入原基因和prolyl-4-hydroxylases (P4H).
- 报告者基因测试 (露西法酶,eGFP) 来确认原体的转录.
- 媒体优化和RNA测序以增强生产并确定降解途径.
- 删除内酶基因protA,以防止原体降解.
主要成果:
- 黑色菌成功产生了部分氧化原蛋白,由序列测序证实.
- 发酵的优化和protA的删除导致了原蛋白度的增加.
- 在摇瓶培养物中实现了5mg/L的化原蛋白的产生.
结论:
- 黑色虫是一种有前途的宿主,可以生产无病毒的氧化原蛋白.
- 这种微生物系统可以帮助满足日益增长的全球对原蛋白的需求.
- 进一步的开发可以将Aspergillus niger作为一个可扩展的原蛋白生产平台.
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