针对OsBBI1的OsSUI1通过调节无意义介导的mRNA衰变途径来负面调节大米免疫力
Hui Wang1,2,3,4, Yuqing Yan1,2,3, Yan Bi1,2,3
1National Key Laboratory for Rice Biology and Breeding, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
The New phytologist
|August 9, 2025
概括
米E3结合酶OsBBI1针对OsSUI1进行降解,激活无意义介导的mRNA衰变 (NMD) 和替代拼接 (AS) 以提高植物对疾病的免疫力.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- E3 泛素连接酶是蛋白质泛素化的关键调节剂,在植物免疫力中起着重要作用.
- 之前已经确定了E3结合酶OsBBI1作为大米爆破抵抗的调节器.
研究的目的:
- 通过识别其相互作用伙伴和下游途径,阐明OsBBI1在水免疫中的调节机制.
- 研究OsBBI1-OsSUI1-无意中介的mRNA衰变 (NMD) -替代拼接 (AS) 网络在抗米病的作用.
主要方法:
- 使用了淘汰和过度表达突变菌株 (ossui1,OsBBI1-OE) 和淘汰突变菌株 (osupf1,osupf3,ossmg7).
- 分析了涉及OsBBI1和OsSUI1.1的蛋白质泛化和降解途径.
- 评估了对大米爆发和细菌爆发病原体的抗病能力.
- 研究了与国防相关的替代拼接转录和NMD因子的积累.
- 研究了丁治疗和真菌感染对基因表达的影响.
主要成果:
- OsBBI1 无处不在并向真核转化启动因子 OsSUI1 进行降解.
- ossui1突变体和OsBBI1-OE植物对爆发和细菌爆发的抗性增强,与防御相关的AS转录增加.
- 无意中介的mRNA衰变 (NMD) 因素 (OsUPF1,OsUPF3,OsSMG7) 在ossui1和OsBBI1-OE植物中被上调,它们的淘汰增强了疾病抵抗力.
- 在ossu1突变体中,基触发的免疫反应被OsSUI1突变部分挽救,这表明OsSUI1在免疫中的作用.
- OsSUI1的OsBBI1-介导降解调节了NMD路径,导致与防御相关的AS转录的积累和增强的水免疫力.
结论:
- OsBBI1-OsSUI1的相互作用启动了一个涉及NMD和AS的信号级联.
- 这个网络微调防御反应,增强大米对病原体的免疫力.
- OsBBI1通过控制OsSUI1水平,作为一个关键的调节者,从而影响NMD和AS通路,以获得强大的疾病抵抗力.
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