关于乙烯碳酸盐作为移动相中的绿色溶剂的使用
Alain Berthod1, Daniel W Armstrong2
1Institute of Analytical Sciences, University of Lyon 1, CNRS, Villeurbanne, France.
Journal of chromatography. A
|August 9, 2025
概括
乙烯碳酸盐 (EC) 作为液体色谱中的绿色溶剂具有前景,当与其他溶剂混合时可以改善分离. 虽然在HILIC和RPLC中有效,但其高沸点限制了检测器的兼容性.
科学领域:
- 绿色化学 绿色化学
- 分析化学 分析化学
- 分离科学 分离科学
背景情况:
- 像碳酸等极性近极性溶剂在液态染色学中被探索为可持续的替代品.
- 乙烯碳酸 (EC),在室温下是固体,需要修改以作为液态染色移动相使用.
研究的目的:
- 研究乙烯碳酸盐 (EC) 在各种液态染色学移动相中作为绿色溶剂的使用.
- 为了确定EC与常见溶剂和其他碳酸 Ester混合时的结点压力.
- 为了评估基于EC的移动相在水友相互作用 (HILIC) 和逆相 (RPLC) 液态染色学中的性能.
主要方法:
- 确定各种溶剂的EC混合物的结点.
- 从实验驾驶压力中使用达西定律估计粘度.
- 评估核化物 (HILIC) 和/芳香 (RPLC) 的分离性能.
- 评估紫外线检测兼容性和梯度性能.
主要成果:
- 可以在液体移动相中使用EC,当其结点通过与其他溶剂混合而降低时.
- 基于EC的移动相改善了核酸的HILIC分离,并使/碳化合物的RPLC成为可能.
- 在EC中,其化强度略低于乙甲.
- 在220nm的紫外线检测是可行的,但梯度化需要去除背景.
结论:
- 乙烯碳酸盐是用于HILIC和RPLC应用的可行的绿色溶剂.
- 它的高沸点对特定的探测器 (例如ELSD) 和电离技术 (例如ESI) 存在局限性.
- 由于EC与基具有有限的溶解性,因此限制了其在正常相染色学中的使用.
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