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Updated: Sep 12, 2025

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在异常性肺纤维化中探索 lncRNAs 的潜在功能:从全转录组测序数据分析的见解

Xinran Ma¹, Dong Chen², Chenghai Li³

¹People's Hospital of Zhengzhou University, Zhengzhou, China.

Clinics (Sao Paulo, Brazil)

|August 9, 2025

概括

长非编码RNAs (lncRNAs) 与异常性肺纤维化 (IPF) 有关. 这项研究确定了541个差异表达的lncRNA,并发现了可能参与IPF病原和炎症的特定lncRNA.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
肺部医学肺部医学

背景情况:

长非编码RNAs (lncRNAs) 越来越多地被认为是它们在疾病中的作用.
在异形性肺纤维化 (IPF) 中 lncRNAs 的特定功能和表达模式在很大程度上仍未被描述.

研究的目的:

研究lncRNAs在IPF病变发生过程中的作用和功能.
确定IPF的潜在诊断生物标志物和治疗点.

主要方法:

在IPF患者和对照组的样本上使用RNA测序 (RNA-seq).
进行差异表达分析以识别差异表达的lncRNAs (DElncRNAs).
采用权重基因协同表达网络分析 (WGCNA) 和单细胞RNA测序 (scRNA-seq) 来探索lncRNA-mRNA相互作用和细胞表达模式.

主要成果:

在IPF中鉴定了541个差异表达的lncRNA (201个上调,340个下调).
在DElncRNAs和差异表达mRNAs (DEmRNAs) 之间发现了显著的相关性,在细胞外基质和炎症反应途径中进行了丰富.
WGCNA强调了与IPF负相关的特定模块 (MEgreen,MEred),揭示了六个关键的lncRNAs (例如FAM13A-AS1,BACH1-IT2) 和它们的共同表达的mRNAs参与炎症.
在IPF样本中的多种细胞类型中,scRNA-seq证实了lncRNA-co-expressedmRNAs的失调表达.

结论:

lncRNAs在IPF中功能性活跃,可能有助于炎症反应.
特定的lncRNAs,如FAM13A-AS1和BACH1-IT2,代表了IPF研究中诊断生物标志物和治疗点的有希望的候选人.

关键词:

共同表达是一种共同表达.异形性肺纤维化症异形性肺纤维化症炎症反应是一种炎症反应.在 LncRNAs 中.在RNA-seqqq.

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