染色素可访问性分析揭示了与番茄果实发育和化相关的功能性cis调节区域
Ying-Juan Cheng1, Yu Zhang1, Jiao Yu1
1College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
The Plant journal : for cell and molecular biology
|August 9, 2025
概括
科学家在番茄果实发育和化过程中绘制了cis-可访问的染色蛋白区域 (cis-ACR). 他们发现cis-ACRs动态变化,并对果实特征作出贡献,结构变异影响其形成.
科学领域:
- 植物基因组学 植物基因组学
- 分子生物学分子生物学
- 发育生物学是发展生物学.
背景情况:
- 非编码DNA调节了对植物发育至关重要的基因表达.
- 染色体可访问性分析识别了非编码DNA中的调控元素.
- 番茄水果的发展和化为研究调节机制提供了一个模型系统.
研究的目的:
- 在番茄果实发育和化过程中定义cis可访问色素区域 (cis-ACRs) 的景观.
- 了解与蛋白质编码DNA相比,cis-ACRs的作用.
- 为了确定调节水果特征的功能性cis-ACRs.
主要方法:
- 使用测序 (ATAC-seq) 检测转化酶可访问的染色体.
- 进行了时间差异分析和栽培番茄和野生番茄之间的比较分析.
- 用基因编辑来验证功能性cis-ACRs.
主要成果:
- ATAC-seq显示了番茄果实发育过程中cis-ACRs的动态变化.
- cis-ACRs对番茄果实化有显著的贡献.
- 基因组结构变异 (SVs) 与栽培番茄中特定cis-ACRs的形成有关.
- 确定了MBP3基因内突中的功能性cis-ACR,它调节了水果的发育和大小.
结论:
- cis-ACRs在调节番茄果实发育和化方面发挥着至关重要的作用.
- 在番茄化过程中,SVs是塑造cis-ACR景观的关键因素.
- 这项研究提供了关于西斯-ACR功能在番茄果生物学的全面观点.
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