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特里克洛桑通过TCF12介导的RASSF8调节抑制人体热囊细胞迁移

Guangzhu Dong¹, Wenbo Huang², Jiahe Qin²

¹Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166, China; Baijiahu Community Health Service Center, Moling Street, Jiangning District, Nanjing, 211102, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|August 9, 2025

概括

通过破坏TCF12-RASSF8通路,克洛桑 (TCS) 暴露会损害 trofhoblast 细胞迁移. 这一发现为TCS生殖毒性和相关并发症的潜在干预提供了洞察力.

科学领域:

生殖毒理学生殖毒理学
细胞和分子生物学是细胞和分子生物学.
环境健康环境健康

背景情况:

一种常见的抗微生物药物克洛桑 (TCS) 与不良的生殖结果有关.
在个人护理产品和医疗消毒剂中广泛使用TCS.
它对人体 trofhoblast 细胞和潜在机制的影响需要阐明.

研究的目的:

研究TCS对热囊细胞的功能影响.
阐明TCS诱导的生殖毒性背后的分子机制.
确定TCS相关生殖问题的潜在治疗目标.

主要方法:

利用HTR-8/SVneo细胞作为人类外性热囊细胞的模型.
应用RNA测序 (RNA-seq) 和ChIP测序 (ChIP-seq) 来分析基因表达和表观遗传变化.
进行了TCF12敲除和露西法酶记者测定,以确认分子相互作用.

主要成果:

暴露于TCS引起了剂量依赖的毒性和受损的 trofhoblast 细胞迁移.
观察到增强器区域 (H3K27ac) 的显著重新分布和转录因子活性 (TCF12) 的改变.
TCS上调调节了TCF12及其目标基因RASSF8,TCF12直接与RASSF8促进体结合.

结论:

确定了一种新的TCF12-RASSF8信号通路,它调解了TCS诱导的热囊细胞迁移障碍.
提供了对Triclosan生殖毒性的分子见解.
突出了TCS诱导的生殖并发症的潜在干预目标.

关键词:

表观遗传调节表观遗传调节在RASSF8的基础上.在TCF12中,三克洛桑是一种三克洛桑.热细胞迁移的迁移

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