对氧化铁纳米颗粒的RNA吸附具有来自核酶的稳定性
Sonali Mohanty1, Mandakini Singh1, Sonali Jena1
1Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India.
International journal of biological macromolecules
|August 9, 2025
概括
氧化铁纳米粒子 (FeONPs) 与RNA相互作用,形成一个保护性的纳米冠状病毒. 这种相互作用改变了RNA结构并增强了对降解的稳定性,显示了RNA治疗的潜力.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
背景情况:
- 纳米颗粒被探索为向药物输送和生物分子相互作用.
- 了解金属纳米粒子-RNA相互作用是推动基于RNA的治疗方法的关键.
研究的目的:
- 为了研究氧化铁纳米粒子 (FeONPs) 和RNA之间的界面相互作用.
- 分析RNA在与FeONPs相互作用时的结构变化.
主要方法:
- 热力学分析用于研究RNA结构转换.
- 接近紫外线的循环二元化,以评估RNA奇拉性和基堆叠的变化.
- 乙化间隔测定试验,以评估RNA结构的可访问性.
- 评估RNase A介导的降解以确定RNA的稳定性.
主要成果:
- 有利的变化表明结构修改和多层RNA吸附到FeONPs上,形成一个纳米冠状病毒.
- 非二态热力学转换和高FeONP度的同质RNA种群表明改变了内部和分子间相互作用.
- 接近紫外线的循环二重化揭示了由于基堆叠而导致的RNA奇拉性变化,减少了乙基化间隔.
- 吸附RNA的FeONP复合体通过硬质阻碍显著地保护RNase A降解.
结论:
- 这项研究阐明了被FeONPs吸附的RNA的结构复杂性.
- 提供了对RNA-FeONP复合体对核酶活性稳定性的洞察.
- 这些发现表明,RNA吸收FeONPs在基于RNA的疗法中具有潜在的应用.
更多相关视频
09:36Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy
Published on: October 19, 2018
9.5K
12:44Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example
Published on: December 3, 2014
54.0K
相关概念视频
RNA Stability
33.9K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.9K
RNA Interference
26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K
RNA Structure
72.4K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
72.4K
RNA Editing
9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K
Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
